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Z380059

Sigma-Aldrich

Breathe-Easy®封膜

Breathe-Easy® sealing membrane
2 of 6 reviewers received a sample product or took part in a promotion

polyurethane membrane with acrylic adhesive pre-cut to fit standard multiwell plates.

别名:

可呼吸封膜, 多孔封膜, 多孔板, 封膜, 微孔板, 微板, 板封, 板封膜, 组织培养, 细胞培养

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100 EA
$322.00

$322.00


请联系客服了解存货情况


选择尺寸

变更视图
100 EA
$322.00

About This Item

分類程式碼代碼:
41122410
NACRES:
NB.22

$322.00


请联系客服了解存货情况

材料

rectangular transparent membrane (polyurethane)

無菌

sterile

特點

piercable

包裝

pack of 100 ea

製造商/商標名

Diversified Biotech BEM-1

相关类别

一般說明

Breathe-Easy ®膜能渗透氧气、二氧化碳和水蒸气,从多孔板中建立各自高度统一的微培养容器。
该膜采用三明治结构。最底层纸衬片保护粘附层,而最上面的载片层保护高度柔性膜外表面,直到它被放置粘到板上。纸衬片也保证了每个单个膜的无菌。粘性密封每一个孔(不像模塑塑料盖片),能够搅动而不会发生交叉污染或微生物渗透。和大多数腐蚀剂和有机溶剂兼容,在 − 80 到 +100℃ 范围内稳定。
注意:推荐使用 Brayer(Z380067 单独出售),保证均匀粘附力并帮助去除最上面的纸衬片。

特點和優勢

Breathe-Easy® 膜:
  • 无菌&非细胞毒性:经伽马辐照灭菌(没有细胞毒性)
  • 防止污染:膜能够避免膜与膜之间的污染,微生物和病毒污染,蒸发(呼吸性膜除外)和样本溢出
  • 完美的大小和设计:6" x 3.25”方形适合于所有微孔板。使用两个刻度结束标签给膜定位,使用方便
  • 热稳定性:能够承受较广的温度范围
  • 简单的样本检索:可刺穿封膜允许通过移液吸头轻松吸除样本

法律資訊

Breathe-Easy is a registered trademark of Diversified Biotech

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Victoria Hsiao et al.
ACS synthetic biology, 4(2), 150-161 (2014-05-23)
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this
Teng Wang et al.
Nature communications, 11(1), 5589-5589 (2020-11-06)
Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid's ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the
Adithi R Varadarajan et al.
NPJ biofilms and microbiomes, 6(1), 46-46 (2020-11-01)
Pseudomonas aeruginosa MPAO1 is the parental strain of the widely utilized transposon mutant collection for this important clinical pathogen. Here, we validate a model system to identify genes involved in biofilm growth and biofilm-associated antibiotic resistance. Our model employs a
Nathan A Sallee et al.
SLAS discovery : advancing life sciences R & D, 25(9), 1047-1063 (2020-07-28)
The identification of novel peptide hormones by functional screening is challenging because posttranslational processing is frequently required to generate biologically active hormones from inactive precursors. We developed an approach for functional screening of novel potential hormones by expressing them in
Filipe Pinto et al.
Nature communications, 11(1), 1529-1529 (2020-04-07)
Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing

Questions

1–4 of 4 Questions  
  1. Is this product optically transparent? Can it be used for luminescence experiments?

    1 answer
    1. This product is distributed on behalf of Diversified Biotech. The films have not been tested for use in luminescence, but are UV transparent to 300 nm.

      Helpful?

  2. Ces films sont-ils adaptés pour de la culture cellulaire? Remplacent-ils le couvercle ou doit-on mettre un film (stérile?) et le couvercle de la plaque dessus?

    1 answer
    1. Yes, these sealing films are sterile and suitable for use in cell culture. When in place, a plate lid is not needed. These films allow for the exchange of oxygen, carbon dioxide, and water vapors while preventing microbial/viral contamination and well-to-well cross contamination.

      Helpful?

  3. Is the Breath-easy sealing meant to be used with or without a lid? does it prevent contamination from leaking in without a lid?

    1 answer
    1. The adhesive seals are meant to be used without a lid. These items provide a secure seal for every well which prevents microbial/viral contamination and well-to-well cross-contamination.

      Helpful?

  4. Is it plate reader friendly for absorbance wavelength

    1 answer
    1. This product is distributed on behalf of Diversified Biotech. The original manufacturer states that these films are UV transparent to 300 nm.

      Helpful?

Reviews

2 of 6 reviewers received a sample product or took part in a promotion
1–3 of 6 Reviews  

Active Filters

    • Review 1
    • Votes 9
    1 out of 5 stars.

    transparent breath easy sealing membranes for microtiter readers

    We bought the Z380059, Breathe-Easy® sealing membrane to measure the growth curve of aerobic bacteria in microtiter plate. From the description of the product it sounds like this is the application they are sold for and in the picture from the producer the membranes look totally transparent. But when we received the product the membranes were rather opaque and could not be used for cell density reading. We contacted the supplier and received this answer: "The manufacturer of Z380059, Breathe-Easy® sealing membrane, has advised that these films are not transparent. Unfortunately we do not have any sealing films that are both breathable and optically clear."

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  1. SF
    • Review 1
    • Votes 2
    5 out of 5 stars.

    superior performance at -80C

    i love this product for its reliable performance, especially at -80 degree C

    Helpful?

    • Review 1
    • Votes 0
    1 out of 5 stars.

    A lot of air bubbles and media leaking out on 384 well plates

    We used the Breath-Easy with a plate lid because we were afraid of contamination. When we came on the next day it was a big mess of media leaking out, air bubbles and definitely cross contamination between the wells. Is the Breath-easy sealing meant to be used without a lid? without contamination risk?

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    Helpful?

    1. Response from MilliporeSigma:

      Breathe-Easy® sealing membranes are intended to prevent cross contamination and do not require use of a lid. When applying the seal, take care to press the adhesive to the cell openings to make sure there is a tight seal on each well.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 3 Ratings-Only Reviews

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