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Xianbao He et al.
PLoS pathogens, 9(8), e1003569-e1003569 (2013-09-07)
The sst1, "supersusceptibility to tuberculosis," locus has previously been shown to be a genetic determinant of host resistance to infection with the intracellular pathogen, Mycobacterium tuberculosis. Chlamydia pneumoniae is an obligate intracellular bacterium associated with community acquired pneumonia, and chronic
Nathan A Baird et al.
PloS one, 3(10), e3376-e3376 (2008-10-15)
Single nucleotide polymorphism (SNP) discovery and genotyping are essential to genetic mapping. There remains a need for a simple, inexpensive platform that allows high-density SNP discovery and genotyping in large populations. Here we describe the sequencing of restriction-site associated DNA
Rawand Masoud et al.
Cell reports. Medicine, 1(8), 100143-100143 (2020-12-10)
Mitochondrial respiration (oxidative phosphorylation, OXPHOS) is an emerging target in currently refractory cancers such as pancreatic ductal adenocarcinoma (PDAC). However, the variability of energetic metabolic adaptations between PDAC patients has not been assessed in functional investigations. In this work, we
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized
Nobuyuki Tanaka et al.
PloS one, 12(4), e0173647-e0173647 (2017-04-06)
The geometrical confinement of small cell colonies gives differential cues to cells sitting at the periphery versus the core. To utilize this effect, for example to create spatially graded differentiation patterns of human mesenchymal stem cells (hMSCs) in vitro or
Minglin Sun et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 27(1), 89-94 (2013-02-23)
To explore the effect of rolling compression loading bioreactor on chondrogenesis of rabbit bone marrow mesenchymal stem cells (BMSCs) with different loading parameters. BMSCs were isolated from New Zealand rabbits, aged 2.5 months. BMSCs at passage 3 were used to
Haruka Koga et al.
Biofabrication, 5(1), 015010-015010 (2013-01-11)
Micropatterning is useful for regulating culture environments. We developed a highly efficient near-infrared-(NIR)-responsive gel and established a new technique that enables cell patterning by NIR irradiation. As a new culture substratum, we designed a tissue culture plate that was coated
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 74(6), 16-23 (2013-01-09)
A scheme of isolation and purification of the enzyme with alpha-L-rhamnosidase activity from culture liquid Cryptococcus albidus 1001 has been developed. It included fractionation by ammonium sulfate and chromatography on TSK-gels Toyopearl HW-60, Fractogel TSK DEAE-650-s and Sepharose 6B. The
Microcasting with agarose gel via degassed polydimethylsiloxane molds for repellency-guided cell patterning
Tanaka N, et al.
Royal Society of Chemistry Advances, 6(60), 54754-54762 (2016)
Gianluca Vadalà et al.
Spine, 38(6), E319-E324 (2013-01-18)
Descriptive anatomical study on ovine and human cadaveric lumbar spinal segments. To describe the alternative transpedicular approach to deliver therapeutic agents into intervertebral disc (IVD). The present delivery approach of therapeutic agents (growth factors/cells/hydrogels) within the IVD is through injection
Purification of recombinant protein derived from the baculovirus expression system using glutathione affinity agarose.
E J Sorscher et al.
Methods in molecular biology (Clifton, N.J.), 39, 337-348 (1995-01-01)
Jia Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(17), 6694-6699 (2013-04-10)
Seamless and minimally invasive integration of 3D electronic circuitry within host materials could enable the development of materials systems that are self-monitoring and allow for communication with external environments. Here, we report a general strategy for preparing ordered 3D interconnected
Shailabh Kumar et al.
Nature communications, 12(1), 6018-6018 (2021-10-16)
Mosquito bites transmit a number of pathogens via salivary droplets deposited during blood-feeding, resulting in potentially fatal diseases. Little is known about the genomic content of these nanodroplets, including the transmission dynamics of live pathogens. Here we introduce Vectorchip, a
Noemi Jiménez-Rojo et al.
Current biology : CB, 30(19), 3775-3787 (2020-08-29)
Sphingolipids play important roles in physiology and cell biology, but a systematic examination of their functions is lacking. We performed a genome-wide CRISPRi screen in sphingolipid-depleted human cells and identified hypersensitive mutants in genes of membrane trafficking and lipid biosynthesis
Lan Zhao et al.
Biomedical chromatography : BMC, 27(9), 1089-1091 (2013-05-18)
To study the leakage at different solution pH values, IgG Sepharose 6FF®, a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which
Protein crystallization in agarose gel with high strength: developing an automated system for protein crystallographic processes
Sugiyama S, et al.
Japanese Journal of Applied Physics, 48(7R), 075502-075502 (2009)
R Alvarez et al.
Journal of virology, 82(1), 471-486 (2007-10-19)
Understanding why human immunodeficiency virus (HIV) preferentially infects some CD4(+) CD45RO(+) memory T cells has implications for antiviral immunity and pathogenesis. We report that differential expression of a novel secreted factor, ps20, previously implicated in tissue remodeling, may underlie why
Emily Ray Ko et al.
Molecular cancer, 7, 77-77 (2008-10-09)
The c-Myb protein is a transcriptional regulator initially identified by homology to the v-Myb oncoprotein, and has since been implicated in human cancer. The most highly conserved portion of the c-Myb protein is the DNA-binding domain which consists of three
Asmini Athman et al.
Plant methods, 10, 29-29 (2014-09-25)
An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These
Jeremy J Flint et al.
NeuroImage, 219, 116997-116997 (2020-06-04)
Since its first description and development in the late 20th century, diffusion magnetic resonance imaging (dMRI) has proven useful in describing the microstructural details of biological tissues. Signal generated from the protons of water molecules undergoing Brownian motion produces contrast
Joshua Schindel et al.
International journal of radiation oncology, biology, physics, 86(2), 387-393 (2013-02-26)
To present a novel marker-flange, addressing source-reconstruction uncertainties due to the artifacts of a titanium intracavitary applicator used for magnetic resonance imaging (MRI)-guided high-dose-rate (HDR) brachytherapy (BT); and to evaluate 7 different MRI marker agents used for interstitial prostate BT
María del Mar Yust et al.
Food science and technology international = Ciencia y tecnologia de los alimentos internacional, 19(3), 217-223 (2013-02-22)
Chickpea protein isolate was hydrolyzed using Flavourzyme immobilized on glyoxyl-agarose beads by multipoint covalent attachment. This Flavourzyme-glyoxyl derivative, produced after 1 h of immobilization at 4 °C followed by 5.5 h at room temperature, presented approximately 51% of the endoprotease activity of Flavourzyme
Jiaqing Yu et al.
Lab on a chip, 20(3), 626-633 (2020-01-11)
Bacterial cellulose (BC), a renewable type of cellulose, has been used in the manufacture of foods, cosmetics, and biomedical products. To produce BC, a high-throughput single-bacterium measurement is necessary to identify the functional bacteria that can produce BC with sufficient
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies.
A Barizien et al.
Journal of the Royal Society, Interface, 16(153), 20180935-20180935 (2019-04-25)
The growth of a cell population from a large inoculum appears deterministic, although the division process is stochastic at the single-cell level. Microfluidic observations, however, display wide variations in the growth of small populations. Here we combine theory, simulations and
S V Sennikov et al.
Journal of immunological methods, 390(1-2), 92-98 (2013-02-08)
Autoantibodies to cytokines are important biological effector molecules that can regulate cytokine activities. The aim of the study was to develop a protocol to purify autoantibodies to tumor necrosis factor from human serum, for use as a calibration material to
Feng Xu et al.
PloS one, 6(4), e19344-e19344 (2011-05-10)
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques
Mostafa Ghannad-Rezaie et al.
Nature communications, 10(1), 2620-2620 (2019-06-15)
Conventional drug screens and treatments often ignore the underlying complexity of brain network dysfunctions, resulting in suboptimal outcomes. Here we ask whether we can correct abnormal functional connectivity of the entire brain by identifying and combining multiple neuromodulators that perturb connectivity
Tobias K H Müller et al.
Journal of chromatography. A, 1285, 97-109 (2013-03-14)
An integrated approach to temperature-controlled chromatography, involving copolymer modified agarose adsorbents and a novel travelling cooling zone reactor (TCZR) arrangement, is described. Sepharose CL6B was transformed into a thermoresponsive cation exchange adsorbent (thermoCEX) in four synthetic steps: (i) epichlorohydrin activation;
Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and
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页码 1 来自 5