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Jeong Ho Hwang et al.
Biochemical and biophysical research communications, 461(2), 427-434 (2015-04-22)
There is no sufficient porcine specific antibody available to investigate the ontogeny and development of porcine pulmonary alveolar macrophage (PAM). Therefore, several mouse anti-porcine macrophage mAbs have been produced and characterized in this study. First, the monoclonal antibody PM18-7, an
Liz O'Donovan et al.
Nucleic acid therapeutics, 25(1), 1-10 (2014-11-21)
We describe two new methods of parallel chemical synthesis of libraries of peptide conjugates of phosphorodiamidate morpholino oligonucleotide (PMO) cargoes on a scale suitable for cell screening prior to in vivo analysis for therapeutic development. The methods represent an extension
Jolon M Dyer et al.
Journal of cosmetic science, 65(1), 25-36 (2014-03-08)
There is increasing awareness of the importance of reductive and oxidative (redox) protein damage in protein-based materials including, hair, wool, nails, and skin. Light-induced damage to protein-based materials is of particular concern because of its impact on age-related degradation and
Nadia Ennaas et al.
Biochemical and biophysical research communications, 462(3), 195-200 (2015-05-03)
Proteins from fish by-product sources are valuable source of bioactive peptides and show promise as functional foods ingredients. The objective of the present study was to isolate and characterize antibacterial peptides from protamex hydrolysates of Atlantic mackerel (Scomber scombrus) by-products.
Sung Hee Ahn et al.
Journal of mass spectrometry : JMS, 48(3), 299-305 (2013-03-16)
In a previous study on matrix-assisted laser desorption ionization (MALDI) of peptides using α-cyano-4-hydroxycinnamic acid (CHCA) as a matrix, we found that the patterns of single-shot spectra obtained under different experimental conditions became similar upon temperature selection. In this paper
Natalia V Gogichaeva et al.
Methods in molecular biology (Clifton, N.J.), 828, 121-135 (2011-11-30)
Here, we describe two different amino acid analysis protocols based on the application of matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry (MS). First protocol describes a MALDI TOF MS-based method for a routine simultaneous qualitative and quantitative analysis
Dong-Hui Xu et al.
International journal of oncology, 45(1), 264-272 (2014-05-03)
Nucleophosmin (NPM, also known as B23), mainly localized in the nucleolus, has been reported to be overexpressed in many types of human cancer, including colon, ovarian, prostate and gastric cancer. NPM was identified while screening the differential nuclear matrix proteins
Christian Secchi et al.
Cellular signalling, 27(4), 777-788 (2015-01-13)
Reactive Oxygen Species (ROS) are crucial to multiple biological processes involved in the pathophysiology of inflammation, and are also involved in redox signaling responses. Although previous reports have described an association between oxidative events and the modulation of innate immunity
Nicholas R Rhodes et al.
Biological trace element research, 148(3), 409-414 (2012-03-07)
Cr(3+), similar to Fe(3+), is transported into cells primarily via endocytosis as the metal-transferrin complex. As Cr(3+) ions are not readily reduced under biological conditions, the ion cannot be transported from endosomes by the same mechanism as iron that utilized
Edyta Burda et al.
Nature communications, 5, 5170-5170 (2014-11-19)
Sensitive detection of small molecule fragments binding to defined sites of biomacromolecules is still a considerable challenge. Here we demonstrate that protein-binding fragments are able to induce enzymatic reactions on the protein surface via dynamic fragment ligation. Fragments binding to
Owen Chan et al.
Diabetes, 62(12), 4239-4246 (2013-08-14)
Suppression of GABAergic neurotransmission in the ventromedial hypothalamus (VMH) is crucial for full activation of counterregulatory responses to hypoglycemia, and increased γ-aminobutyric acid (GABA) output contributes to counterregulatory failure in recurrently hypoglycemic (RH) and diabetic rats. The goal of this
Zheng Tan et al.
The Journal of biological chemistry, 290(1), 46-55 (2014-11-20)
There has been fast growing evidence showing that glycolysis plays a critical role in the activation of immune cells. Enhanced glycolysis leads to increased formation of intracellular lactate that is exported to the extracellular environment by monocarboxylate transporter 4 (MCT4).
Stano Pekár et al.
Die Naturwissenschaften, 101(7), 533-540 (2014-06-01)
It is rare to find a true predator that repeatedly and routinely kills prey larger than itself. A solitary specialised ant-eating spider of the genus Zodarion can capture a relatively giant prey. We studied the trophic niche of this spider
B Minea et al.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 34(2), 367-383 (2014-09-17)
This is the first multi-centre study regarding yeast infections in Romania. The aim was to determine the aetiological spectrum and susceptibility pattern to fluconazole, voriconazole and the novel compound MXP-4509. The 551 isolates were identified using routine laboratory methods, matrix-assisted
Ajay Kumar et al.
Anti-cancer drugs, 24(2), 158-171 (2012-08-31)
In the present investigation, we explored the molecular mechanisms of the tumoricidal action of α-cyano-4-hydroxycinnamate (αCHC) on the cells of Dalton's lymphoma (DL), which is a murine T cell lymphoma. In-vitro treatment of the DL cells with αCHC resulted in
Simona Barnini et al.
BMC microbiology, 15, 124-124 (2015-06-19)
Rapid identification of the causative agent(s) of bloodstream infections using the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) methodology can lead to increased empirical antimicrobial therapy appropriateness. Herein, we aimed at establishing an easier and simpler method, further referred to as the
Akikazu Yasuda et al.
European journal of mass spectrometry (Chichester, England), 19(1), 29-37 (2013-07-12)
The electrical conductivity of the matrix crystal might be a new factor to enhance matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) sensitivity. In MALDI-MS, several compounds are used as a standard matrix. Utilization of such compounds is based on an a
Rita Casadonte et al.
Analytical and bioanalytical chemistry, 407(18), 5323-5331 (2015-05-04)
Amyloidosis is a heterogeneous group of protein misfolding diseases characterized by deposition of amyloid proteins. The kidney is frequently affected, especially by immunoglobulin light chain (AL) and serum amyloid A (SAA) amyloidosis as the most common subgroups. Current diagnosis relies
Sadanori Sekiya et al.
Rapid communications in mass spectrometry : RCM, 26(6), 693-700 (2012-02-14)
3-Aminoquinoline/α-cyano-4-hydroxycinnamic acid (3AQ/CHCA) is a liquid matrix (LM), which was reported by Kumar et al. in 1996 for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It is a viscous liquid and has some advantages of durability of ion generation by a
Vera Miranda-Gonçalves et al.
Neuro-oncology, 15(2), 172-188 (2012-12-22)
Gliomas exhibit high glycolytic rates, and monocarboxylate transporters (MCTs) play a major role in the maintenance of the glycolytic metabolism through the proton-linked transmembrane transport of lactate. However, their role in gliomas is poorly studied. Thus, we aimed to characterize
Jürgen Borlak et al.
BMC genomics, 16, 124-124 (2015-04-16)
Hepatocellular carcinoma (HCC) is on the rise and the sixth most common cancer worldwide. To combat HCC effectively research is directed towards its early detection and the development of targeted therapies. Given the fact that epidermal growth factor (EGF) is
Hans R Aerni et al.
Nucleic acids research, 43(2), e8-e8 (2014-11-08)
The genetic code can be manipulated to reassign codons for the incorporation of non-standard amino acids (NSAA). Deletion of release factor 1 in Escherichia coli enhances translation of UAG (Stop) codons, yet may also extended protein synthesis at natural UAG
Zhiqiang Zhao et al.
Cancer letters, 342(1), 150-158 (2013-09-10)
Monocarboxylate transporter isoform 1 (MCT1) is an important member of the proton-linked MCT family and has been reported in an array of human cancer cell lines and primary human tumors. MCT1 expression is associated with developing a new therapeutic approach
Andrew D Shubin et al.
Tissue engineering. Part A, 21(11-12), 1733-1751 (2015-03-13)
More than 40,000 patients are diagnosed with head and neck cancers annually in the United States with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of
David Bonnel et al.
Analytical biochemistry, 434(1), 187-198 (2012-11-13)
In the current study, we compared plastic matrix-assisted laser desorption/ionization (MALDI) plates pre-spotted with different solid ionic matrices. Data reflect that after 3 months of storage, the standards were oxidized in α-cyano-4-hydroxycinnamic acid (HCCA) whether or not in HCCA/3-acetylpyridine (3APY)
Arturo Roca-Rivada et al.
Scientific reports, 5, 12214-12214 (2015-07-23)
In the context of obesity, strong evidences support a distinctive pathological contribution of adipose tissue depending on its anatomical site of accumulation. Therefore, subcutaneous adipose tissue (SAT) has been lately considered metabolically benign compared to visceral fat (VAT), whose location
Ubaldo E Martinez-Outschoorn et al.
Cell cycle (Georgetown, Tex.), 11(21), 3956-3963 (2012-10-23)
We have previously suggested that ketone body metabolism is critical for tumor progression and metastasis. Here, using a co-culture system employing human breast cancer cells (MCF7) and hTERT-immortalized fibroblasts, we provide new evidence to directly support this hypothesis. More specifically
Suzanne Gaudet et al.
BMC cell biology, 12, 55-55 (2011-12-22)
The human homologue of the Drosophila Discs-large tumor suppressor protein, hDlg, is a multi-domain cytoplasmic protein that localizes to the membrane at intercellular junction sites. At both synaptic junctions and epithelia cell-cell junctions, hDlg is known to recruit several signaling
Irene Riz et al.
Methods in molecular biology (Clifton, N.J.), 699, 431-447 (2010-12-01)
In vivo biotinylation tagging, based on a method in which a protein of interest is tagged with a peptide that is biotinylated in vivo by coexpression of Escherichia coli BirA biotin ligase, has been successfully used for the isolation of
Xiaobai Li et al.
International journal of molecular sciences, 15(11), 19877-19897 (2014-11-05)
The labellum in orchids shares homology with the inner lateral petals of the flower. The labellum is a modified petal and often distinguished from other petals and sepals due to its large size and irregular shape. Herein, we combined two-dimensional
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