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基于显色法的微生物鉴定
对于微生物学家而言,最基础的染色方法是由丹麦细菌学家Hans Christian Gram在1884年开发的。
原代细胞培养基础知识
什么是原代细胞?关于动物和人类原代细胞系和培养基的全面概述,以及它们如何应用于生物医学细胞培养应用。相对于细胞系,原代培养物的均一性较低。
离心分离
差速离心法和密度梯度离心法是两种离心式颗粒分离技术。CsCl梯度离心法(氯化铯离心法)用来制备从DNA中密度梯度离心分离RNA的溶液。
细胞培养表面的演变
为了使得细胞可以良好地附着,疏水的聚苯乙烯表面必须被改造为更亲水的表面。在含有血清的培养基中发现的细胞附着蛋白(玻连蛋白和纤连蛋白)可被黏附在容器底部并扩散,从而提供了更适于细胞附着的表面
Apoptosis Assays
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Centrifugation Separations
Differential centrifugation and density gradient centrifugation are two types of centrifugal techniques for separating particles.CsCl gradient centrifugation, or Caesium chloride centrifugation is used to make solutions for the separation of RNA from DNA by density gradient centrifugation.
Identification of Microorganisms Based on Color
For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.
Cell Migration & Invasion Assays
Metastasis is the cumulative result of multiple changes in tumor cells. Explore tumor metastasis employing various cell migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay and in vitro scratch assay.
Applications of Quantum Dots in Bioimaging and Bioassays
Professor Xiaohu Gao (University of Washington, USA) provides a overview of recent quantum dot (QD) advancements and their potential for advancing bioassay and bioimaging technologies.