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磷酸二酯酶类
环核苷酸磷酸二酯酶 (PDEs) 催化 cAMP 和/或 cGMP 的水解。存在有11种不同的哺乳动物PDE家族。
酶法测定淀粉酶活性(淀粉酶活性测定)
使用淀粉酶活性测定试剂盒比色检测生物样品中淀粉酶活性的测定实验方案。
如何使用溶于硫酸铵悬液的酶
以硫酸铵悬浮液形式提供的酶的使用指南
G蛋白(异源三聚体)
由α,β和γ亚基所组成的异源三聚体G蛋白通过调节细胞内效应蛋白如酶和离子通道,可对由活化的七跨膜(7TM)受体产生的细胞外信号产生响应。
EC编号
用于代谢研究的酶的完整EC编号列表。
HMG-CoA Reductase (HMGR) Assay Kit
3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.
Protein Kinase C (PKC)
Protein kinase C (PKC) is an AGC kinase that phosphorylates serine and threonine residues in many target proteins.
Neurotensin Receptors
Neurotensin is a 13 amino acid regulatory peptide found mainly in gut and brain. Intestinal neurotensin is produced by a discrete population of endocrine cells (N cells) scattered throughout the jejuno-ileal mucosa.
G Proteins (Heterotrimeric)
Heterotrimeric G proteins, comprising α, β and γ subunits, respond to extracellular signals generated by activated seven-transmembrane (7TM) receptors by modulating intracellular effector proteins such as enzymes and ion channels.
Nuclear Receptors (PPARs)
Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that are related to retinoid, steroid and thyroid hormone receptors.
Glycobiology - Carbohydrate Enzymes and Proteins
Explore our complex carbohydrate proteins, enzyme products and kits for investigating oligosaccharides, polysaccharides, and numerous additional aspects of your carbohydrate workflow.
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
Enzymatic Method for Determining Amylase Activity (Amylase Activity Assay)
Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.
β Secretase (BACE1) Activity Assay for BACE1 Inhibitor Screening
In recent years, the biological processes involved in the pathogenesis of Alzheimer’s disease (AD) have become increasingly better understood. It is now recognized that a key role is played by BACE1 proteases that cleave amyloid precursor protein (APP) to produce
Enzymatic Assay of Chitosanase from Streptomyces Species
To standardize a procedure for determining the enzymatic activity of Chitosanase.
Enzymatic Assay of Cathepsin B
This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.
Phosphodiesterases
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and/or cGMP. There are 11 different mammalian PDE families.
Phospholipase D
Phosphatidylcholine (PC)-specific phospholipase D (PLD) enzymes catalyze the hydrolysis of PC to generate choline and phosphatidic acid. These enzymes belong to a superfamily of phosphatidyltransferases with a characteristic unique duplicated catalytic domain motif, the PLD or " HKD"
Phospholipase C
The hydrolysis of a minor membrane phospholipid, phosphatidylinositol 4,5-bisphosphate (PIP2) by a specific phospholipase C (PLC) is one of the earliest key events in the regulation of various cell functions by more than 100 extracellular signaling molecules.
Enzymatic Assay of Proteinase K with Hemoglobin Substrate
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
Neuropeptidases
We offers many products related to neuropeptidases for your research needs.
Compound Library Screening with LOPAC®1280 Small Molecules
Learn about compound library screening and explore our compound libraries, including our Library of Pharmacologically Active Compounds (LOPAC®1280) portfolio to experience the gold-standard collection of biologically annotated, ready-to-screen compounds.
Mucins, Glycosylated Proteins, Properties & Diagram
Mucins are a diverse family of densely glycosylated proteins. Mucin domains within the protein core are rich in threonine, serine and hydroxyproline enabling post-translational O-glycosylation.
Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)
Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.
Horseradish Peroxidase (HRP) Enzymes
Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.
Tubulin
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells.
Aprotinin
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Coenzyme A
Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.
Complement Proteins
Application Index: Complement Proteins offered by Sigma-alderich online.The complement system is a complex cascade involving proteolytic cleavage of serum glycoproteins often activated by cell receptors. This cascade ultimately results in induction of the inflammatory response, phagocyte chemotaxis and opsonization, and
Cholesterol Esterification
Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.