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使用X-tremeGENE HP转染试剂进行慢病毒生产
慢病毒是一种用于在研究应用中转染多种细胞类型的强大工具。
CRISPR / Cas核酸酶RNA介导的基因组编辑
了解CRISPR Cas9、定义和工作原理。CRISPR是一种全新、实惠的基因组编辑工具,可让所有人掌握基因组编辑。
慢病毒转导实验方案
如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导,以实现稳定的长期沉默和表型变化的详细操作步骤。
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors
Antibiotic Optimization for Cell Transduction Using a Cytotoxicity Profile
Experimentally determine the appropriate antibiotic concentration for selecting stable cell lines. This titration protocol can determine the lowest concentration of puromycin needed to efficiently select transduced cells.