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Functional genomics screening

facet applications:Functional genomics screening
facet content type:Protocol
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使用X-tremeGENE HP转染试剂进行慢病毒生产
CRISPR / Cas核酸酶RNA介导的基因组编辑
了解CRISPR Cas9、定义和工作原理。CRISPR是一种全新、实惠的基因组编辑工具,可让所有人掌握基因组编辑。
如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导,以实现稳定的长期沉默和表型变化的详细操作步骤。
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Antibiotic Optimization for Cell Transduction Using a Cytotoxicity Profile
Experimentally determine the appropriate antibiotic concentration for selecting stable cell lines. This titration protocol can determine the lowest concentration of puromycin needed to efficiently select transduced cells.