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Gel electrophoresis
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使用抗体 Atto 染料缀合物进行荧光多重检测
免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。
Auto2D®全自动双向凝胶电泳仪
Auto2D®双向电泳仪全面自动化，将复杂的的双向电泳转变为一种快速、简单和可重现的技术。Auto2D®可在不到2小时内，高重现性定位复杂蛋白。
蛋白电泳染色剂选择指南
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
限制性内切核酸酶 — 分子剪
术语“限制酶”源自Werner Arber和Matthew Meselson实验室对肠杆菌噬菌体λ（λ噬菌体）的研究。
选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准
选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
DNA/蛋白质电泳及故障排除表
DNA和蛋白质凝胶电泳(SDS-PAGE)应用中的疑难问题解答。提供琼脂糖凝胶中DNA的有效分离范围和加样染料的DNA迁移大小。
mPAGE® Lux SDS-PAGE制胶系统
了解可在3分钟制得SDS-PAGE即用凝胶的mPAGE® Lux制胶系统。可视需要现用现制SDS-PAGE凝胶。
DNA / Protein Electrophoresis and Troubleshooting Tables
DNA / Protein Electrophoresis and Troubleshooting Tables
Components of Rehydration Solution
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Protein Staining Reagents Selection Guide
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Water for Protein Electrophoresis & Western Blotting
Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
mPAGE®Lux Casting System for SDS-PAGE Gels
Refresh your gel casting workflow with ready-to-use SDS-PAGE gels in just 3 minutes. Discover faster, simpler, and safer gel casting methods.
Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Sample Prep & Gel Electrophoresis Troubleshooting
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Troubleshooting 2-D DIGE Results
Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.
Auto2D® Gel Electrophoresis Device
Auto2D® automates difficult 2D electrophoresis methods for protein localization with high reproducibility in under two hours.
Bis Tris Polyacrylamide Gel Electrophoresis Technology
Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.