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Digital PCR
数位PCR是终点PCR方法,其用于绝对定量和针对相似多数序列的背景分析少数序列,例如体细胞突变的定量。
逆转录实验方案(一步法SYBR Green I染料检测)
逆转录(RT)是指使用逆转录酶和dNTP将RNA转化为cDNA的过程。
REDTaq™应用
介绍RedTaq的应用和优势,包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。
逆转录实验方案
将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。
终点 PCR:特异性和产量增强的抗体介导的热启动 PCR 方案
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.
REDExtract-N-Amp™组织PCR试剂盒实验方案
exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法,可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。
SEQR – SeqPlex RNA扩增试剂盒实验方案
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法,
SeqPlex 增强型DNA扩增试剂盒 (SEQXE)实验方案
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
标准逆转录方案(两步法)
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战,例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。
寡核苷酸解链温度
使用最邻近法或基础法等理论方法对寡核苷酸的解链温度(Tm)进行计算。DNA Tm是指50%寡核苷酸与其完美互补链构成双链体,而另外50%游离于溶液中的温度。
FFPE 组织
存档的福尔马林固定和石蜡包埋 (FFPE) 组织样本是分析基因表达和研究多种疾病的宝贵资源。
完整全转录组扩增试剂盒实验方案(WTA2)
WTA2(一种全转录组扩增(WTA)方法)能够在区区4小时内,代表性地扩增出纳克级的总RNA而没有3'偏差。
使用Jumpstart™Taq DNA聚合酶(D9307)扩增DNA
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.
寡核苷酸退火实验方案
退火是加热和冷却两条具有互补序列的单链寡核苷酸的过程。热量会破坏所有氢键,而冷却则会在序列之间形成新的键。
REDExtract-N-Amp™ 植物PCR试剂盒实验方案
Description:REDExtract-N-Amp植物PCR试剂盒含有预混液形式的惰性上样染料,可在扩增后轻松凝胶上样。本实验方案提供了一种采用优化的预混液来快速提取扩增用植物基因组DNA的操作方法。
Plant-Based PCR Inhibitors During DNA Extraction and PCR Master Mix Performance
Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.
Long and Accurate PCR Amplification of DNA with RedAccuTaq®
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA
End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Reverse Transcription Protocol
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Selecting Correctly Expressing Recombinants
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
REDExtract-N-Amp Plant PCR Kits Protocol
The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves.
Whole Genome Amplification: Extraction Protocol - Blood Card
Whole Genome Amplification: Extraction Protocol - Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream analysis. This protocol provides
Whole Blood
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield.
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Reverse Transcription Protocol Using SYBR Green Dye Detection
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
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