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DNA寡核苷酸合成
了解我们用于DNA寡核苷酸生产的寡核苷酸固相亚磷酰胺合成法的步骤。
寡核苷酸质量控制和质量保证
Sigma制造流程的基础在于一套稳健的质量管理体系,其推动了向ISO 9001:2008认证的合规性。
定量聚合酶链反应和数字聚合酶链反应的检测方法
荧光染料或探针包含在PCR混合物中,用以监测在反应进行过程中DNA扩增子浓度的变化。
Polymerase Chain Reaction:
聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成:变性、退火和延伸
PCR技术指南
基本PCR/qPCR/dPCR实验方案的部分实例可用作探索本指南理论章节所述某些概念的基础。除了提供更一般的实验方案为适应特定的基于PCR的研究奠定良好的实践基础外,还包括用于检测方法质量控制的详细实验方案。
PCR/qPCR数据分析
传统的PCR完成后,会通过琼脂糖凝胶——最近更常使用的是毛细管电泳系统——来分辨并分析数据。
ThermaGenix产品概述
如何在PCR中摆脱非特异性条带和脱靶引物。
使用PCR
一般考虑因素;RT-PCR中要考虑的因素;防止残留污染;故障排除
分子克隆的问题解决
分子克隆是将目的基因 (GOI) 插入到质粒载体中的过程,然后将该载体插入到表达 GOI 编码的蛋白质的细胞中。一旦蛋白质在细胞中得到表达,这种表达即可用于各种不同的研究(如细胞信号传导、形态学或其他方面)。
简介和历史时间表
了解聚合酶链反应(PCR)的历史,从促进它的发现的基本原理到获得诺贝尔化学奖,以及实时PCR(qPCR)和数字PCR等最近的发展。
锁核酸
锁核酸 (LNA) 常见问题解答
寡核苷酸定量
凭借高度偶联的性质,DNA可吸收紫外光。因此可通过紫外分光光度计轻松对DNA进行定量。
PCR 实验优化和验证
PCR测定指南可指导您全程了解引物验证和其他测定优化因素,确保实现理想的DNA / RNA定量高灵敏度和特异性。
RT-PCR / RT-qPCR故障排除指南
制定PCR或RT-PCR / RT-qPCR故障排除方案以确保数据可靠,这一工作至关重要。RT-PCR或PCR错误和问题的潜在来源包括操作员人为错误、PCR预混液和寡核苷酸设计。本PCR故障排除指南概述并详细介绍了RT-PCR方案的解决办法。
维甲酸与基因表达
全反式维甲酸(RA, ATRA)是一种多效激活因子,负责调节与正常脊椎动物细胞分化、细胞增殖、凋亡和胚胎发育等过程相关的基因。
Polymerase Chain Reaction
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
KAPA2G Fast Multiplex Kits FAQs
Frequently asked questions (FAQs) for KAPA2G Fast Multiplex Kits
MIQE – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Our qPCR services, including primer and probe designs, assay protocol development, troubleshooting, and data analysis support, adhere to MIQE, which allows you to publish or bring your product to market faster and with confidence.
Hot Start PCR
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
What is Hot Start PCR?
Learn about the benefits of using Hot Start PCR reagents to suppress enzymatic activity before PCR reaction tubes enter the thermal cycler.
PCR Assay Optimization and Validation
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Locked Nucleic Acid
Frequently asked questions about Locked Nucleic Acids (LNA)
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
Optimizing Crude Plant Samples for PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
GC-RICH PCR System Troubleshooting
GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
ThermaStop™ PCR Additive
PCR additive added to master mix that stops nonspecific bands after cool down.
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