Merck
Search Within
Applications
Content Type
Applied Filters:
Applications:Protein labeling and modification
Content Type:Protocol
Showing 1-19 of 19 results
Sort by Relevance
用于核酸去磷酸化的小牛肠(CIP)碱性磷酸酶
CIP可用于脱去DNA、RNA及核糖和脱氧核糖核苷三磷酸上的5’-磷酸基团。如何进行DNA去磷酸化的详细实验方案。
聚糖的质谱
配备有高分辨率和质量准确性的现代质谱(MS)设备的发展已使其可用于分析聚糖以进行图谱和结构研究。
肽文库设计工具教程
为您的目标天然蛋白设计重叠的肽片段文库。该肽文库设计工具的功能包括设置自定义序列参数(肽长度和氨基酸补偿)、格式化序列输出并将自定义的肽文库序列导出到其他程序(Microsoft Word、Excel等)。
酶法去糖基化
酶法去糖基化策略:从多糖中连续水解单个单糖可用于阐明多糖组分的结构和功能。
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Enzymatic Deglycosylation
Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.
Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
Neuraminidase (Sialidase) Protocol
Using Neuraminidase to remove sialic acid from purified lipooligosaccharide (LOS)
Conjugating Alkaline Phosphatase to Antibodies and Other Proteins
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.
Purification or Removal of Biotin and Biotinylated Substances
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.
Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads
This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Tag Removal by Enzymatic Cleavage
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Peptide Quick Tips
Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.
Coupling Protocol for Primary Amine of a Ligand
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
N-Glycosidase F Protocol & Troubleshooting
N-Glycosidase F Protocol & Troubleshooting
Mass Spectrometry of Glycans
The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies.
Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Coupling to Microparticles
Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.