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Protein labeling and modification
facet applications:Protein labeling and modification
facet content type:Technical Article
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肽偶联剂选择指南
Novabiochem®可提供大量的偶联剂用于原位活化。原位活化试剂易于使用 - 即使是利用空间位阻氨基酸,并且它们的使用通常没有副反应。
自组装单层:烷硫醇的优点
我们提供了一篇关于自组装单层的文章:烷硫醇的优点。
近红外(NIR) 荧光染料
NIR荧光染料
N-连接聚糖概述
N-连接聚糖、修饰和降解
糖基化磷脂酰肌醇锚定糖蛋白概述
GPI锚定糖蛋白
聚糖标记
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时,它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因,它们在细胞表面的表现通常被称为“糖”。
用于抗体表征的酶
抗体现今是治疗药物中成长最快的类别
肽聚糖的结构、生物合成和功能
肽聚糖(PGN)的基本结构含有N-乙酰葡糖胺(GlcNAc)与N-乙酰胞壁酸交替单元的碳水化合物主链,其中N-乙酰胞壁酸残基与肽交联。
肽修饰:N-末端、内部、和C-末端
肽修饰:N-末端、内部、和C-末端
金纳米颗粒:性质和应用
金(Au)纳米颗粒具有可调的光学和电子性质,适用于许多应用,包括光伏、传感器、药物递送和催化。
Ni-NTA-Atto共轭物
Ni-NTA-Atto共轭物为 His 标记的融合蛋白提供特异性和高灵敏度的检测。
固相合成
多肽是采用固相FMOC或BOC化学法在PEG-聚苯乙烯载体树脂上合成的。合成完成后,先去除侧链保护基团,同时从树脂上切割多肽。随后,将经切割和去保护的多肽材料沉淀、清洗并溶解在含H2O/ACN/HOAC的缓冲液中,然后进行冻干处理。
用于固相肽合成的ChemMatrix®树脂
ChemMatrix®是PCAS BioMatrix专有的一种基于100%PEG(聚乙二醇)的树脂。
用于不对称合成的手性胺
手性胺已广泛用于不对称合成,例如用作对映选择性去质子化反应中的手性碱,或用于拆分消旋的酸。
O-连接糖蛋白回顾
O-连接糖蛋白通常是分子量>200kDa的大蛋白。糖基化通常发生在高密度簇上,并可占总量的50-80%。
Near-infrared (NIR) Fluorescence Dyes
NIR Fluorescence Dyes
EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Chymotrypsin
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Chiral Amines in Asymmetric Synthesis
Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.
ChiPros Chiral Amines
Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.
N-Linked Glycans Overview
N-linked glycosylation, modification, and degradation
Glycoprotein Deglycosylation
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Glycosylphosphatidylinisotol-anchored Glycoproteins Overview
GPI Anchored Glycoproteins
Post Translational Modification
Protein modifications are crucial for disease study. Analysis methods are key.
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