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Applications:Protein lysis and extraction
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TRI Reagent® 实验方案
TRI试剂是一种单步RNA分离试剂,可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法,以及各种样品提取方法和故障排除方案。
WB检测样本前处理:细胞裂解和蛋白提取
了解免疫印迹样品制备方法,以进行细胞裂解和从培养的组织和细胞中高效提取蛋白质。
Liberase™ TH Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend. Select the Liberase Enzyme Blend most similar to your tissue dissociation application, or
cOmplete™, Mini, EDTA-free Protocol
Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.
Liberase™ TL Research Grade Protocol & Troubleshooting
Liberase™ TL Research Grade Protocol & Troubleshooting
Nuclear Protein Extraction Without the Use of Detergent
Detergents can interfere with the labeling efficiency of the extracted proteins. Therefore, a procedure that does not include the use of detergents should be used for the preparation of nuclear proteins.
TRI Reagent® Protocol
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
Protease Inhibition Using Protease Inhibitor Mix
Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Handling Inclusion Bodies in Recombinant Protein Expression
How to isolate proteins from inclusion bodies using Cytiva products.
Liberase™ DL Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Cell Lysis and Protein Extraction for Western Blotting
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.