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使用Stabilyser™试剂稳定组织提取物中的RNA和蛋白质
稳定完全、均匀的组织匀浆。使用Stabilyser试剂维持RNA和功能蛋白质的稳定性,它是在一种均一裂解物混合物中保护核酸和活性蛋白完整性的唯一一种试剂。
酵母细胞裂解试剂
我们的酵母细胞裂解试剂具有通用性、无磷酸盐、非变性等特点。CelLytic Y细胞裂解试剂可作为酵母细胞裂解和蛋白溶解的有效方法。CelLytic Y酵母酶解试剂盒为从酿酒酵母、巴斯德毕赤酵母和粟酒裂殖酵母酵母菌株中高效形成原生质球和提取蛋白提供了一种便捷的方法。
裂解酶
裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程:只需加入酶,将试管放入水浴然即可。但这过程中到底发生了什么?
去垢剂的性质和应用
在细胞裂解、膜蛋白和脂质增溶、蛋白结晶或蛋白质免疫印迹应用中更有效地使用去垢剂。
BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria
Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit
cOmplete™ Lysis-M Protocol & Troubleshooting
cOmplete™ Lysis-M Inhibitor: Low protein concentration in lysate
Roche Protein Stabilization Reagents
Get maximum protection after protein isolation by using protease and phosphatase inhibitor cocktail tablets from Roche.
Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria
The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.
Lysing Enzymes
Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your tube in the water-bath and walk away, but what is actually going on in that
Protease Inhibitor Cocktails
Protease Inhibitor Cocktails maintain and preserve cellular protein composition following cell lysis. When used with CelLytics, they also preserve target protein activity. These cocktails have been optimized for a wide variety of specific applications, ensuring excellent inhibition and ease of
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
RNA Stabilization and Protein Stabilization in Tissue Extracts with Stabilyser™ Reagent
Stabilize complete, uniform tissue homogenates. Maintain RNA and functional proteins with Stabilyser reagent, the only reagent that protects the integrity of nucleic acid and active protein in one uniform lysate mixture.
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
Simple, efficient extraction of protein from hepatocytes with CytoBuster™ Reagent
CytoBuster Protein Extraction Reagent is a formulation of detergents prepared for the efficient extraction of soluble proteins from mammalian cells
Organelle Isolation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
Phosphatase Inhibitor Cocktail
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad
BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
Yeast Cell Lysis Reagents
Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for
Benzonase® Nuclease Q&A
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.