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色谱柱填充和抗体亲和层析的制备
本页介绍了高效的色谱柱填充和抗体亲和层析的制备。
离心分离
差速离心法和密度梯度离心法是两种离心式颗粒分离技术。CsCl梯度离心法(氯化铯离心法)用来制备从DNA中密度梯度离心分离RNA的溶液。
Sera-Mag™ 和 Sera-Mag SpeedBeads 磁性颗粒
Sera-Mag 和 Sera-Mag SpeedBeads 为分子生物学应用、核酸分离和研究免疫分析提供了高成本效益的磁珠分离技术。
Column Packing and Preparation for Affinity Chromatography of Antibodies
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Centrifugation Separations
Differential centrifugation and density gradient centrifugation are two types of centrifugal techniques for separating particles.CsCl gradient centrifugation, or Caesium chloride centrifugation is used to make solutions for the separation of RNA from DNA by density gradient centrifugation.
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Agarose Beads Vs. Magnetic Beads in ChIP
Agarose beads Vs. Magnetic beads in Chromatin Immunoprecipitation (ChIP)
Immunoprecipitation Techniques
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
Robust, load independent viral clearance in monoclonal antibody purification with Natrix® Q chromatography membrane
Membrane adsorbers are a cost-effective, robust, single-use solution for monoclonal antibody polishing
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Centrifugation Basics
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles
Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.