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Hirotsugu Hiramatsu et al.
Biochemistry, 52(13), 2371-2380 (2013-03-21)
The pH dependence of the β-galactoside binding activity of human galectin-1 (hGal-1) was investigated by fluorescence spectroscopy using lactose as a ligand. The obtained binding constant Kb was 2.94 ± 0.10 mM(-1) at pH 7.5. The Kb value decreased at
Ronald F Parsons et al.
Current opinion in organ transplantation, 19(2), 100-107 (2014-02-21)
To update the reader on the recent literature in liver, kidney, pancreas, and intestine static cold preservation, and to identify which solutions are most advantageous for each organ. The comparison of randomized trials of histidine-tryptophan-ketoglutarate (HTK), Celsior, and University of
Madhavan Narayanan et al.
The Journal of biological chemistry, 288(20), 14310-14319 (2013-04-02)
NADH:ubiquinone oxidoreductase (complex I) pumps protons across the membrane using downhill redox energy. The Escherichia coli complex I consists of 13 different subunits named NuoA-N coded by the nuo operon. Due to the low abundance of the protein and some
Neha Garg et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(18), 7258-7263 (2013-04-17)
The biosynthesis of several classes of ribosomally synthesized and posttranslationally modified peptides involves dehydration of serine and threonine residues. For class I lantibiotics, thiopeptides, and goadsporin, this dehydration is catalyzed by lanthionine biosynthetic enzyme B (LanB) or LanB-like proteins. Although
Griet Van Zeebroeck et al.
Nature chemical biology, 5(1), 45-52 (2008-12-09)
Transporter-related nutrient sensors, called transceptors, mediate nutrient activation of signaling pathways through the plasma membrane. The mechanism of action of transporting and nontransporting transceptors is unknown. We have screened 319 amino acid analogs to identify compounds that act on Gap1
Juleane Lunardi et al.
Current topics in medicinal chemistry, 13(22), 2866-2884 (2013-10-12)
Worldwide, tuberculosis is the leading cause of morbidity and mortality due to a single bacterial pathogen, Mycobacterium tuberculosis (Mtb). The increasing prevalence of this disease, the emergence of multi-, extensively, and totally drug-resistant strains, complicated by co-infection with the human
Ritesh Kumar et al.
Biochemistry, 53(13), 2112-2125 (2014-03-15)
Hemophores from Pseudomonas aeruginosa (HasAp), Serratia marcescens (HasAsm), and Yersinia pestis (HasAyp) bind hemin between two loops. One of the loops harbors conserved axial ligand Tyr75 (Y75 loop) in all three structures, whereas the second loop (H32 loop) contains axial
Edward A Berry et al.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 13(4), 481-498 (2008-04-18)
Early investigation of the electron paramagnetic resonance spectra of bis-histidine-coordinated membrane-bound ferriheme proteins led to the description of a spectral signal that had only one resolved feature. These became known as "highly anisotropic low-spin" or "large g(max)" ferriheme centers. Extensive
Maryam Rezazadeh et al.
Analytica chimica acta, 773, 52-59 (2013-04-09)
In the present work, pulsed electromembrane extraction (PEME) was performed for the first time, as a new concept of electrically enhanced microextraction method, for extraction and quantification of histidine, phenylalanine and tryptophan in different matrices. PEME offers an alternative to
Yipin Wu et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 309(7), R747-R756 (2015-08-01)
Inwardly rectifying potassium channels play essential roles in renal physiology across phyla. Barium-sensitive K(+) conductances are found on the basolateral membrane of a variety of insect Malpighian (renal) tubules, including Drosophila melanogaster. We found that barium decreases the lumen-positive transepithelial
Shoji Yamada et al.
Biochimica et biophysica acta, 1840(1), 434-442 (2013-10-15)
Nα-Acetylhistidine (NAH) is present in very high concentrations exclusively in the brain and lens of ectothermic vertebrates, including ray-finned fishes, amphibians and reptiles, and not in those of endothermic birds and mammals. Although NAH is known to be synthesized from
Related (βα)8-barrel proteins in histidine and tryptophan biosynthesis: a paradigm to study enzyme evolution.
Felix List et al.
Chembiochem : a European journal of chemical biology, 12(10), 1487-1494 (2011-06-10)
Alessio Innocenti et al.
Bioorganic & medicinal chemistry letters, 19(13), 3430-3433 (2009-05-26)
An activation study of the membrane-associated carbonic anhydrase (CA, EC isoform XV with a series of natural and non-natural amino acids and aromatic/heterocyclic amines is reported. Murine CA XV was strongly activated by some amino acids (d-Phe, l-/d-DOPA, d-Trp
V G Doroshenko et al.
Prikladnaia biokhimiia i mikrobiologiia, 49(2), 149-154 (2013-06-26)
Strain MG 1655+hisGr hisL'-Delta, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal modifications of the laboratory Escherichia coli strain MG 1655+, which has a known genome sequence. A feedback-resistant ATP-phosphoribosyl
Sandeep Kumar et al.
Protein and peptide letters, 19(6), 657-662 (2012-04-24)
Soybean urease has been investigated extensively to reveal the presence of histidine residue (s) in the active site and their potential role in the catalysis. The spectrophotometric studies using diethylpyrocarbonate (DEP) showed the modification of 11.76 ± 0.1 histidine residues
Saroj Kumar Amar et al.
Toxicology letters, 239(3), 182-193 (2015-10-07)
Solar UV radiation is main factor of photocarcinogenesis, photoageing, and phototoxicity; thus, protection from UV radiation is major concern. Sunscreens containing UV filters are suggested as sun safe practices, but safety of UV filters remains in controversies. Benzophenone-1 (BP1) is
Darío Ortiz de Orué Lucana et al.
The Journal of biological chemistry, 289(49), 34214-34228 (2014-10-25)
The extracellular protein HbpS from Streptomyces reticuli interacts with iron ions and heme. It also acts in concert with the two-component sensing system SenS-SenR in response to oxidative stress. Sequence comparisons suggested that the protein may bind a cobalamin. UV-visible
Pelin Onsekizoglu Bagci et al.
Journal of food science, 80(9), N2071-N2078 (2015-08-05)
Gold nanoparticles (AuNPs) were synthesized at room temperature following a simple, rapid, and green route using fresh-squeezed apple juice as a reducing reagent. The optimal AuNPs, based on the particle color, stability, and color change suitable for colorimetric detection of
Gerson F Duarte Junior et al.
Electrophoresis, 36(16), 1935-1940 (2015-03-27)
This paper describes the use of ionic solutions as sensing electrodes for capacitively coupled contactless conductivity detection on electrophoresis microchips. Initially, two channels were engraved in a PMMA holder by using a CO2 laser system and sealed with a thin
Danielle M Williamson et al.
The Journal of biological chemistry, 288(26), 19154-19165 (2013-05-09)
The proprotein convertase furin requires the pH gradient of the secretory pathway to regulate its multistep, compartment-specific autocatalytic activation. Although His-69 within the furin prodomain serves as the pH sensor that detects transport of the propeptide-enzyme complex to the trans-Golgi
Tomas F M Kuijpers et al.
The FEBS journal, 280(23), 6184-6195 (2013-10-12)
Sulfite salts are widely used as antibrowning agents in food processing. Nevertheless, the exact mechanism by which sulfite prevents enzymatic browning has remained unknown. Here, we show that sodium hydrogen sulfite (NaHSO3) irreversibly blocks the active site of tyrosinase from
Justyna Borowska et al.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 20(6), 989-1004 (2015-07-19)
This work is focused on the study of DNA binding and cleavage properties of 2'-deoxyadenosines modified with ester/amide of histidine (his(6)dA ester, his(6)dA amide) and their copper(II) complexes. To determine the coordination mode of the complex species potentiometric and spectroscopic
Michael Sippach et al.
Biochimica et biophysica acta, 1838(7), 1760-1768 (2014-03-04)
The conformational dynamics of the histidine ABC transporter HisQMP2 from Salmonella enterica serovar Typhimurium, reconstituted into liposomes, is studied by site-directed spin labeling and double electron-electron resonance spectroscopy in the absence of nucleotides, in the ATP-bound, and in the post-hydrolysis
Pang-Hung Hsu et al.
PloS one, 10(8), e0135174-e0135174 (2015-08-14)
The budding yeast Saccharomyces cerevisiae has recently been described as an emerging opportunistic fungal pathogen. Fungal cell wall mannoproteins have been demonstrated to be involved in adhesion to inert surfaces and might be engaged in virulence. In this study, we
Daniela Vullo et al.
Bioorganic & medicinal chemistry letters, 18(15), 4303-4307 (2008-07-17)
An activation study of the human carbonic anhydrase (hCA, EC isoforms hCA III (cytosolic) and IV (membrane-associated) with a series of natural and non-natural amino acids and aromatic/heterocyclic amines is reported. hCA III was efficiently activated by d-His, serotonin
Dan Zhao et al.
The Analyst, 140(24), 8157-8164 (2015-11-17)
A novel, highly sensitive and selective dual-readout (colorimetric and fluorometric) sensor based on fluorescent carbon dots (CDs) and unmodified gold nanoparticles (AuNPs) for the detection of thiocyanate (SCN(-)) was proposed. Amino-functionalized CDs could be readily adsorbed onto the surface of
Isao Nishimori et al.
Bioorganic & medicinal chemistry, 15(15), 5351-5357 (2007-05-15)
The secretory isozyme of human carbonic anhydrase (hCA, EC, hCA VI, has been cloned, expressed, and purified in a bacterial expression system. The kinetic parameters for the CO(2) hydration reaction proved hCA VI to possess a k(cat) of 3.4
Claudia Hernández-Mosqueira et al.
Biochimica et biophysica acta, 1850(12), 2485-2496 (2015-09-24)
Alkaline phosphatases are dimeric hydrolytic enzymes that dephosphorylate nucleotides and proteins. AP-TNAP is found primarily in skeletal tissues were it plays a major role in the mineralization of the extracellular matrix and bone formation. In this study we found through
Marie-Rose Abdo et al.
Bioorganic & medicinal chemistry letters, 19(9), 2440-2443 (2009-04-07)
Activation of the human carbonic anhydrase (CA, EC isozymes I, II (cytosolic) and IX (transmembrane, tumor-associated isoform) with a series of arylsulfonylhydrazido-l-histidines incorporating 4-substituted-phenyl, pentafluorophenyl- and beta-naphthyl moieties was investigated. The compounds showed a weak hCA I activation profile
Saroj Kumar Amar et al.
Toxicology letters, 235(2), 84-95 (2015-03-25)
Sunscreen users have been increased, since excessive sun exposure increased the risk of skin diseases. Benzophenone (BP) and its derivatives are commonly used in sunscreens as UV blocker. Its photosafety is concern for human health. Our study showed the role
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