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Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge.

Lance M Hellman et al.

Methods (San Diego, Calif.), 54(1), 31-38 (2010-12-29)

Many recombinant proteins carry an oligohistidine (His(X))-tag that allows their purification by immobilized metal affinity chromatography (IMAC). This tag can be exploited for the site-specific attachment of chromophores and fluorophores, using the same metal ion-nitrilotriacetic acid (NTA) coordination chemistry that

Semiquinone and cluster N6 signals in His-tagged proton-translocating NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli.

Madhavan Narayanan et al.

The Journal of biological chemistry, 288(20), 14310-14319 (2013-04-02)

NADH:ubiquinone oxidoreductase (complex I) pumps protons across the membrane using downhill redox energy. The Escherichia coli complex I consists of 13 different subunits named NuoA-N coded by the nuo operon. Due to the low abundance of the protein and some

Preservation solutions for static cold storage of abdominal allografts: which is best?

Ronald F Parsons et al.

Current opinion in organ transplantation, 19(2), 100-107 (2014-02-21)

To update the reader on the recent literature in liver, kidney, pancreas, and intestine static cold preservation, and to identify which solutions are most advantageous for each organ. The comparison of randomized trials of histidine-tryptophan-ketoglutarate (HTK), Celsior, and University of

Bis-histidine-coordinated hemes in four-helix bundles: how the geometry of the bundle controls the axial imidazole plane orientations in transmembrane cytochromes of mitochondrial complexes II and III and related proteins.

Edward A Berry et al.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 13(4), 481-498 (2008-04-18)

Early investigation of the electron paramagnetic resonance spectra of bis-histidine-coordinated membrane-bound ferriheme proteins led to the description of a spectral signal that had only one resolved feature. These became known as "highly anisotropic low-spin" or "large g(max)" ferriheme centers. Extensive

Eric R. Braverman

The Healing Nutrients Within: Facts, Findings, and New Research on Amino Acids (2003)

Two inwardly rectifying potassium channels, Irk1 and Irk2, play redundant roles in Drosophila renal tubule function.

Yipin Wu et al.

American journal of physiology. Regulatory, integrative and comparative physiology, 309(7), R747-R756 (2015-08-01)

Inwardly rectifying potassium channels play essential roles in renal physiology across phyla. Barium-sensitive K(+) conductances are found on the basolateral membrane of a variety of insect Malpighian (renal) tubules, including Drosophila melanogaster. We found that barium decreases the lumen-positive transepithelial

Studies of histidine residues in soybean (Glycine max) urease.

Sandeep Kumar et al.

Protein and peptide letters, 19(6), 657-662 (2012-04-24)

Soybean urease has been investigated extensively to reveal the presence of histidine residue (s) in the active site and their potential role in the catalysis. The spectrophotometric studies using diethylpyrocarbonate (DEP) showed the modification of 11.76 ± 0.1 histidine residues

Molecularly imprinted polymer dedicated to the extraction of glyphosate in natural waters.

K Puzio et al.

Journal of chromatography. A, 1361, 1-8 (2014-08-26)

Three molecularly imprinted polymers (MIPs) have been synthesized in order to bind efficiently glyphosate (GLY) in natural waters (mineral and underground). Since the target analyte is polar and hydrophilic, electrostatic interactions and hydrogen bonds have been favored with two templates

Exploiting the interactions between poly-histidine fusion tags and immobilized metal ions.

Wen-Hui K Kuo et al.

Biotechnology letters, 33(6), 1075-1084 (2011-02-15)

Immobilized metal affinity chromatography (IMAC) of proteins containing poly-histidine fusion tags is an efficient research tool for purifying recombinant proteins from crude cellular feedstocks at laboratory scale. Nevertheless, to achieve successful purification of large amounts of the target protein for

Carbonic anhydrase activators: activation of human isozymes I, II and IX with phenylsulfonylhydrazido l-histidine derivatives.

Marie-Rose Abdo et al.

Bioorganic & medicinal chemistry letters, 19(9), 2440-2443 (2009-04-07)

Activation of the human carbonic anhydrase (CA, EC 4.2.1.1) isozymes I, II (cytosolic) and IX (transmembrane, tumor-associated isoform) with a series of arylsulfonylhydrazido-l-histidines incorporating 4-substituted-phenyl, pentafluorophenyl- and beta-naphthyl moieties was investigated. The compounds showed a weak hCA I activation profile

Metalless electrodes for capacitively coupled contactless conductivity detection on electrophoresis microchips.

Gerson F Duarte Junior et al.

Electrophoresis, 36(16), 1935-1940 (2015-03-27)

This paper describes the use of ionic solutions as sensing electrodes for capacitively coupled contactless conductivity detection on electrophoresis microchips. Initially, two channels were engraved in a PMMA holder by using a CO2 laser system and sealed with a thin

A Simple and Green Route for Room-Temperature Synthesis of Gold Nanoparticles and Selective Colorimetric Detection of Cysteine.

Pelin Onsekizoglu Bagci et al.

Journal of food science, 80(9), N2071-N2078 (2015-08-05)

Gold nanoparticles (AuNPs) were synthesized at room temperature following a simple, rapid, and green route using fresh-squeezed apple juice as a reducing reagent. The optimal AuNPs, based on the particle color, stability, and color change suitable for colorimetric detection of

Targeting the histidine pathway in Mycobacterium tuberculosis.

Juleane Lunardi et al.

Current topics in medicinal chemistry, 13(22), 2866-2884 (2013-10-12)

Worldwide, tuberculosis is the leading cause of morbidity and mortality due to a single bacterial pathogen, Mycobacterium tuberculosis (Mtb). The increasing prevalence of this disease, the emergence of multi-, extensively, and totally drug-resistant strains, complicated by co-infection with the human

Replacing the axial ligand tyrosine 75 or its hydrogen bond partner histidine 83 minimally affects hemin acquisition by the hemophore HasAp from Pseudomonas aeruginosa.

Ritesh Kumar et al.

Biochemistry, 53(13), 2112-2125 (2014-03-15)

Hemophores from Pseudomonas aeruginosa (HasAp), Serratia marcescens (HasAsm), and Yersinia pestis (HasAyp) bind hemin between two loops. One of the loops harbors conserved axial ligand Tyr75 (Y75 loop) in all three structures, whereas the second loop (H32 loop) contains axial

Highly Efficient Photocatalytic Hydrogen Production of Flower-like Cadmium Sulfide Decorated by Histidine.

Qizhao Wang et al.

Scientific reports, 5, 13593-13593 (2015-09-05)

Morphology-controlled synthesis of CdS can significantly enhance the efficiency of its photocatalytic hydrogen production. In this study, a novel three-dimensional (3D) flower-like CdS is synthesized via a facile template-free hydrothermal process using Cd(NO3)2•4H2O and thiourea as precursors and L-Histidine as

Histidine methylation of yeast ribosomal protein Rpl3p is required for proper 60S subunit assembly.

Qais Al-Hadid et al.

Molecular and cellular biology, 34(15), 2903-2916 (2014-05-29)

Histidine protein methylation is an unusual posttranslational modification. In the yeast Saccharomyces cerevisiae, the large ribosomal subunit protein Rpl3p is methylated at histidine 243, a residue that contacts the 25S rRNA near the P site. Rpl3p methylation is dependent upon

Carbonic anhydrase activators. Activation of the membrane-associated isoform XV with amino acids and amines.

Alessio Innocenti et al.

Bioorganic & medicinal chemistry letters, 19(13), 3430-3433 (2009-05-26)

An activation study of the membrane-associated carbonic anhydrase (CA, EC 4.2.1.1) isoform XV with a series of natural and non-natural amino acids and aromatic/heterocyclic amines is reported. Murine CA XV was strongly activated by some amino acids (d-Phe, l-/d-DOPA, d-Trp

Screening of Non- Saccharomyces cerevisiae Strains for Tolerance to Formic Acid in Bioethanol Fermentation.

Cyprian E Oshoma et al.

PloS one, 10(8), e0135626-e0135626 (2015-08-19)

Formic acid is one of the major inhibitory compounds present in hydrolysates derived from lignocellulosic materials, the presence of which can significantly hamper the efficiency of converting available sugars into bioethanol. This study investigated the potential for screening formic acid

Photoinduced aggregation of a model antibody-drug conjugate.

Gregory M Cockrell et al.

Molecular pharmaceutics, 12(6), 1784-1797 (2015-04-17)

During synthesis, purification, and especially storage, antibody-drug conjugates (ADCs) may be exposed to various types of light. Several of the drugs commonly conjugated to antibodies contain photosensitive functional groups. Exposure to light could generate an excited state of the drug

Comprehensive cytotoxic evaluation of morin, a bioflavonoid against verapamil on rat gastrointestinal epithelium for novel pharmaceutical application involving P-glycoprotein inhibition.

Gurunath Surampalli et al.

The Journal of pharmacy and pharmacology, 67(8), 1083-1099 (2015-04-14)

In this study, a comprehensive and comparative cytotoxic evaluation of morin against verapamil on rat intestinal epithelium as P-gp inhibitors through in-vitro gastrointestinal short-term toxicity assays involving permeability studies for safety evaluation was investigated. In this study, the effect of

Transport and signaling via the amino acid binding site of the yeast Gap1 amino acid transceptor.

Griet Van Zeebroeck et al.

Nature chemical biology, 5(1), 45-52 (2008-12-09)

Transporter-related nutrient sensors, called transceptors, mediate nutrient activation of signaling pathways through the plasma membrane. The mechanism of action of transporting and nontransporting transceptors is unknown. We have screened 319 amino acid analogs to identify compounds that act on Gap1

The extracellular heme-binding protein HbpS from the soil bacterium Streptomyces reticuli is an aquo-cobalamin binder.

Darío Ortiz de Orué Lucana et al.

The Journal of biological chemistry, 289(49), 34214-34228 (2014-10-25)

The extracellular protein HbpS from Streptomyces reticuli interacts with iron ions and heme. It also acts in concert with the two-component sensing system SenS-SenR in response to oxidative stress. Sequence comparisons suggested that the protein may bind a cobalamin. UV-visible

[The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants].

V G Doroshenko et al.

Prikladnaia biokhimiia i mikrobiologiia, 49(2), 149-154 (2013-06-26)

Strain MG 1655+hisGr hisL'-Delta, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal modifications of the laboratory Escherichia coli strain MG 1655+, which has a known genome sequence. A feedback-resistant ATP-phosphoribosyl

Evaluation of spin freezing versus conventional freezing as part of a continuous pharmaceutical freeze-drying concept for unit doses.

L De Meyer et al.

International journal of pharmaceutics, 496(1), 75-85 (2015-05-20)

Spin-freezing as alternative freezing approach was evaluated as part of an innovative continuous pharmaceutical freeze-drying concept for unit doses. The aim of this paper was to compare the sublimation rate of spin-frozen vials versus traditionally frozen vials in a batch

An ectotherm homologue of human predicted gene NAT16 encodes histidine N-acetyltransferase responsible for Nα-acetylhistidine synthesis.

Shoji Yamada et al.

Biochimica et biophysica acta, 1840(1), 434-442 (2013-10-15)

Nα-Acetylhistidine (NAH) is present in very high concentrations exclusively in the brain and lens of ectothermic vertebrates, including ray-finned fishes, amphibians and reptiles, and not in those of endothermic birds and mammals. Although NAH is known to be synthesized from

Involvement of histidine residues in the pH-dependent β-galactoside binding activity of human galectin-1.

Hirotsugu Hiramatsu et al.

Biochemistry, 52(13), 2371-2380 (2013-03-21)

The pH dependence of the β-galactoside binding activity of human galectin-1 (hGal-1) was investigated by fluorescence spectroscopy using lactose as a ligand. The obtained binding constant Kb was 2.94 ± 0.10 mM(-1) at pH 7.5. The Kb value decreased at

Role of type I & type II reactions in DNA damage and activation of caspase 3 via mitochondrial pathway induced by photosensitized benzophenone.

Saroj Kumar Amar et al.

Toxicology letters, 235(2), 84-95 (2015-03-25)

Sunscreen users have been increased, since excessive sun exposure increased the risk of skin diseases. Benzophenone (BP) and its derivatives are commonly used in sunscreens as UV blocker. Its photosafety is concern for human health. Our study showed the role

A review of phylogenetic and metabolic relationships between the acylamino acids, N-acetyl-L-aspartic acid and N-acetyl-L-histidine, in the vertebrate nervous system.

M H Baslow

Journal of neurochemistry, 68(4), 1335-1344 (1997-04-01)

N-Acetyl-L-histidine (NAH) and N-acetyl-L-aspartic acid (NAA) are major constituents of vertebrate brain and eye with distinct phylogenetic distributions. They are characterized by high tissue concentrations, high tissue/extracellular fluid gradients, and a continuous regulated efflux into the extracellular fluid. As a

Related (βα)8-barrel proteins in histidine and tryptophan biosynthesis: a paradigm to study enzyme evolution.

Felix List et al.

Chembiochem : a European journal of chemical biology, 12(10), 1487-1494 (2011-06-10)

A single amino acid in VP2 is critical for the attachment of infectious bursal disease subviral particles to immobilized metal ions and DF-1 cells.

Su-Yuan Lai et al.

Biochimica et biophysica acta, 1844(7), 1173-1182 (2014-04-16)

VP2 protein is the primary host-protective immunogen of infectious bursal disease virus (IBDV). His249 and His253 are two surface histidine residues in IBDV subviral particles (SVP), which is formed by twenty VP2 trimers when the VP2 protein of a local

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