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m2643
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Woo-Yong Lee et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(38), 13936-13941 (2014-09-11)
CXCR6-GFP(+) cells, which encompass 70% invariant natural killer T cells (iNKT cells), have been found primarily patrolling inside blood vessels in the liver. Although the iNKT cells fail to interact with live pathogens, they do respond to bacterial glycolipids presented
José F Huertas-Pérez et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 32(8), 1352-1357 (2015-07-01)
A rapid, simple and effective method for the determination of citrinin in rice by UHPLC coupled to fluorescence detection has been developed and validated. Extraction of citrinin from rice samples was achieved by applying a QuEChERS-based extraction/partitioning process with water
Lilia C De Jesus et al.
American journal of obstetrics and gynecology, 212(1), 94-94 (2014-07-22)
Antenatal magnesium (anteMg) is used for various obstetric indications including fetal neuroprotection. Infants exposed to anteMg may be at risk for respiratory depression and delivery room (DR) resuscitation. The study objective was to compare the risk of acute cardiorespiratory events
Myles Robert Minter et al.
Journal of neuroinflammation, 12, 71-71 (2015-04-17)
Neuro-inflammation has long been implicated as a contributor to the progression of Alzheimer's disease in both humans and animal models. Type-1 interferons (IFNs) are pleiotropic cytokines critical in mediating the innate immune pro-inflammatory response. The production of type-1 IFNs following
Xiaosan Wu et al.
BMC cardiovascular disorders, 13, 5-5 (2013-01-25)
Atrial arrhythmia (AA) is the most common complication after coronary artery bypass grafting (CABG). Only beta-blockers and amiodarone have been convincingly shown to decrease its incidence. The effectiveness of magnesium on this complication is still controversial. This meta-analysis was performed
Peter Schuff-Werner et al.
British journal of haematology, 162(5), 684-692 (2013-07-03)
Pseudothrombocytopenia remains a challenge in the haematological laboratory. The pre-analytical problem that platelets tend to easily aggregate in vitro, giving rise to lower platelet counts, has been known since ethylenediamine-tetra acetic acid EDTA and automated platelet counting procedures were introduced
Renata Bocian et al.
Hippocampus, 25(11), 1393-1406 (2015-03-31)
Previous in vivo data suggested that orexin neuropeptides (ORX(A) and ORX(B) ) synthetized in hypothalamic neurons were involved in the mechanism of generation of the hippocampal formation theta rhythm. Surprisingly, this suggestion has never been directly proved by experiments using
Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase.
Zakiya Whatley et al.
G3 (Bethesda, Md.), 5(6), 1301-1311 (2015-04-17)
The dnaQ gene of Escherichia coli encodes the ε subunit of DNA polymerase III, which provides the 3' → 5' exonuclease proofreading activity of the replicative polymerase. Prior studies have shown that loss of ε leads to high mutation frequency
Xin Zheng et al.
Journal of virology, 89(9), 4786-4797 (2015-01-30)
Angiopoietin-1 (ANGPT-1) is a secreted glycoprotein that was first characterized as a ligand of the Tie2 receptor. In a previous study using microarray analysis, we found that the expression of ANGPT-1 was upregulated in Kaposi's sarcoma-associated herpesvirus (KSHV)-infected primary effusion
Xi Zhan et al.
Pharmaceutical research, 32(7), 2280-2291 (2015-01-17)
A key step of delivering extracellular agents to its intracellular target is to escape from endosomal/lysosomal compartments, while minimizing the release of digestive enzymes that may compromise cellular functions. In this study, we examined the intracellular distribution of both fluorecent
Anna Gadaj et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 960, 105-115 (2014-05-06)
An UHPLC-MS/MS method was developed for the quantitative confirmatory analysis of residues of nitroimidazole drugs (dimetridazole, ipronidazole, metronidazole, ornidazole and ronidazole) and their corresponding hydroxy metabolites (HMMNI, ipronidazile-OH and metronidazole-OH) in aquaculture tissue. Samples were extracted by shaking in acetonitrile
Sharmistha Samanta Koruri et al.
Archives of microbiology, 197(7), 955-963 (2015-07-15)
The present investigation deals with the optimization of cell growth rate of the candidate probiotic Pediococcus acidilactici in the presence of the specific prebiotic inulin. Three independent variables viz. concentration of inulin, concentration of glucose and pH have been selected
Sung-Hyun Kang et al.
Naunyn-Schmiedeberg's archives of pharmacology, 388(7), 773-780 (2015-02-17)
Intravesical administration of the cytotoxic drug doxorubicin is a common treatment for superficial carcinoma of the bladder, but it is associated with significant urological adverse effects. The aim of this study was to identify doxorubicin-induced changes in the local mechanisms
Yi Wang et al.
International journal of urology : official journal of the Japanese Urological Association, 21(10), 1059-1064 (2014-05-23)
To investigate the effect of diabetes on urothelial modulation of bladder contractility. Bladder strips (urothelium intact or denuded) were prepared from 8-week-old streptozotocin-induced diabetic (n = 19) and non-diabetic control rats (n = 10). The effect of modulators of MaxiK (iberiotoxin and tetraethylammonium) and
Yuko Matsushita et al.
Journal of microbiological methods, 112, 11-20 (2015-03-01)
Variation in band position between gels is a well-known problem in denaturing gradient gel electrophoresis (DGGE). However, few reports have evaluated the degree of variation in detail. In this study, we investigated the variation in band positions of DNA samples
Michalina Oplatowska-Stachowiak et al.
Journal of agricultural and food chemistry, 63(43), 9441-9451 (2015-10-10)
There are more than 300 potential mycotoxins that can contaminate food and feed and cause adverse effects in humans and animals. The data on the co-occurrence of mycotoxins in novel animal feed materials, such as distiller's dried grain with solubles
Junxue Wu et al.
Journal of environmental management, 161, 11-20 (2015-07-06)
In this study, the removal of fosthiazate in an aqueous solution using zero valent iron (ZVI) and the related removal reaction mechanism were investigated. The results indicate that the dissipation of fosthiazate adheres to a pseudo-first order reaction law. The
Judith C A Cluitmans et al.
PloS one, 10(5), e0125580-e0125580 (2015-05-02)
Panthothenate kinase-associated neurodegeneration (PKAN) belongs to a group of hereditary neurodegenerative disorders known as neuroacanthocytosis (NA). This genetically heterogeneous group of diseases is characterized by degeneration of neurons in the basal ganglia and by the presence of deformed red blood
Peter Donndorf et al.
Interactive cardiovascular and thoracic surgery, 19(2), 211-217 (2014-05-07)
Closed minimal extracorporeal circulation (MECC) systems currently do not represent the standard of surgical care for open-heart surgery. Yet, considering the beneficial results reported for coronary artery bypass graft (CABG) surgery, we used an MECC system in aortic valve replacement
Lei Yang et al.
AMB Express, 4, 54-54 (2014-11-18)
Aspergillus carbonarius has potential as a cell factory for the production of different organic acids. At pH 5.5, A.carbonarius accumulates high amounts of gluconic acid when it grows on glucose based medium whereas at low pH, it produces citric acid.
D R Scott et al.
Alimentary pharmacology & therapeutics, 42(11-12), 1315-1326 (2015-10-02)
The gastric H(+) ,K(+) -ATPase is the preferred target for acid suppression. Until recently, the only drugs that effectively inhibited this ATPase were the proton pump inhibitors (PPIs). PPIs are acid-activated prodrugs that require acid protection. Once acid-activated, PPIs bind
M Javier Herrero-Turrión et al.
BMC genomics, 15, 874-874 (2014-10-09)
A great number of studies have investigated changes induced by morphine exposure in gene expression using several experimental models. In this study, we examined gene expression changes during chronic exposure to morphine during maturation and differentiation of zebrafish CNS. Microarray
Sumihiro Koyama et al.
Marine biotechnology (New York, N.Y.), 17(5), 678-692 (2015-08-06)
The purpose of this study was to develop a novel electrical retrieval method (ER method) for living sponge-associated microorganisms from marine sponges frozen at -80 °C. A -0.3-V vs. Ag/AgCl constant potential applied for 2 h at 9 °C induced
Fuchao Xu et al.
Journal of chromatography. A, 1401, 33-41 (2015-05-23)
A multi-residue analytical method was developed for the determination of a range of flame retardants (FRs), including polybrominated diphenyl ethers (PBDEs), emerging halogenated FRs (EFRs) and organophosphate FRs (PFRs), in food matrices. An ultrasonication and vacuum assisted extraction (UVAE), followed
D H Currie et al.
Applied and environmental microbiology, 80(16), 5001-5011 (2014-06-08)
Thermoanaerobacterium saccharolyticum, a Gram-positive thermophilic anaerobic bacterium, grows robustly on insoluble hemicellulose, which requires a specialized suite of secreted and transmembrane proteins. We report here the characterization of proteins secreted by this organism. Cultures were grown on hemicellulose, glucose, xylose
Frans J Smit et al.
European journal of medicinal chemistry, 90, 33-44 (2014-12-03)
A series of dihydroartemisinyl-chalcone esters were synthesized through esterification of chalcones with dihydroartemisinin (DHA). The hybrids were screened against chloroquine (CQ) sensitive (3D7) and CQ resistant (W2) strains of intraerythrocytic Plasmodium falciparum parasites, and were all found to be active
Nidhi B Panda et al.
Journal of clinical anesthesia, 25(2), 92-97 (2013-01-22)
To study the minimal effective dose of magnesium sulfate to control blood pressure (BP) during intubation in hypertensive patients. Prospective, randomized, double-blind study. Operating room of an academic medical center. 80 adult, ASA physical status 1 and 2, controlled hypertensive
Naoki Tanigawa et al.
PloS one, 9(11), e113345-e113345 (2014-11-19)
Polymerase chain reaction (PCR) amplification of multiple templates using common primers is used in a wide variety of molecular biological techniques. However, abundant templates sometimes obscure the amplification of minor species containing the same primer sequences. To overcome this challenge
Ranjan Devkota et al.
Bio-protocol, 8(13), e2913-e2913 (2018-07-05)
FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans. Using FRAP, we have shown that cold, glucose and
Zdenka Veprikova et al.
Journal of agricultural and food chemistry, 63(29), 6633-6643 (2015-07-15)
Mycotoxin contamination of dietary supplements represents a possible risk for human health, especially in the case of products intended for people suffering from certain health conditions. The aim of this study was to assess the extent of this problem based
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