Merck

Targeted delivery of polyoxometalate nanocomposites.

Small (Weinheim an der Bergstrasse, Germany) (2011-09-29)
Georg Geisberger, Susann Paulus, Emina Besic Gyenge, Caroline Maake, Greta R Patzke
ABSTRACT

Polyoxometalate/carboxymethyl chitosan nanocomposites with an average diameter of 130 nm are synthesized and labeled with fluorescein isothiocyanate (FITC) for a combined drug-carrier and cellular-monitoring approach. [Eu(β(2) -SiW(11) O(39) )(2) ](13-) /CMC nanospheres as a representative example do not display cytotoxicity for POM concentrations up to 2 mg mL(-1) . Cellular uptake of fluoresecently labelled {EuSiW(11) O(39) }/FITC-CMC nanoparticles is monitored with confocal laser scanning microscopy. Nanoparticle uptake occurs after incubation times of around 1 h and no cyctotoxic effects are observed upon prolonged treatment. The preferential location of the POM/CMC nanocomposites in the perinuclear region is furthermore verified with transmission electron microscopy investigations on unlabeled nanoparticles. Therefore, this approach is a promising dual strategy for the safe cellular transfer and monitoring of bioactive POMs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
羧甲基纤维素 钠盐, low viscosity
Sigma-Aldrich
羧甲基纤维素 钠盐, Medium viscosity
Sigma-Aldrich
羧甲基纤维素 钠盐, High viscosity
Sigma-Aldrich
羧甲基纤维素 钠盐, medium viscosity
Sigma-Aldrich
羧甲基纤维素 钠盐, ultra high viscosity, highly purified