Merck
  • Home
  • Search Results
  • An integrated workflow for charting the human interaction proteome: insights into the PP2A system.

An integrated workflow for charting the human interaction proteome: insights into the PP2A system.

Molecular systems biology (2009-01-22)
Timo Glatter, Alexander Wepf, Ruedi Aebersold, Matthias Gstaiger
ABSTRACT

Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP-MS studies on human protein complexes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
乙腈, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
乙腈, anhydrous, 99.8%
Sigma-Aldrich
乙腈, HPLC Plus, ≥99.9%
Sigma-Aldrich
乙腈, for HPLC, for UV, ≥99.9% (GC)
Sigma-Aldrich
乙腈, ACS reagent, ≥99.5%
Sigma-Aldrich
2,2,2-三氟乙醇, ReagentPlus®, ≥99%
Sigma-Aldrich
乙腈, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
[Glu1]-纤维蛋白肽B 人, ≥90% (HPLC)
Sigma-Aldrich
乙腈, suitable for HPLC-GC, ≥99.8% (GC)
Sigma-Aldrich
乙腈, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
2,2,2-三氟乙醇, BioUltra, for molecular biology, ≥99.0% (GC)
Supelco
乙腈, analytical standard
Sigma-Aldrich
乙腈, biotech. grade, ≥99.93%
Supelco
2,2,2-三氟乙醇, analytical standard, for NMR spectroscopy
Sigma-Aldrich
乙腈, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
乙腈, ReagentPlus®, 99%
Sigma-Aldrich
乙腈, suitable for DNA synthesis, ≥99.9% (GC)
Sigma-Aldrich
乙腈, ≥99.5% (GC)