Merck
  • Home
  • Search Results
  • Screening and identification of newly isolated cellulose-degrading bacteria from the gut of xylophagous termite Microcerotermes diversus (Silvestri).

Screening and identification of newly isolated cellulose-degrading bacteria from the gut of xylophagous termite Microcerotermes diversus (Silvestri).

Mikrobiologiia (2013-04-25)
Z Pourramezan, G R Ghezelbash, B Romani, S Ziaei, A Hedayatkhah
ABSTRACT

The aim of the present study was to isolate and characterize the cellulose-degrading bacteria from the gut of the local termite, Microcerotermes diversus (Silvestri), inhabiting the Khuzestan province of Iran. The microorganisms capable of growing in the liquid medium containing cellulose as the only source of carbon were isolated and their cellulolytic activity on CMC-containing media was confirmed by the congo red clearing zone assay. The isolates were identified based on biochemical characteristics and the phylogenetic analysis of 16S rRNA gene fragments. The results of the present study show that three cellulose-degrading bacteria isolated from local termite guts belonged to the genera Acinetobacter, Pseudomonas and Staphylococcus and four cellulose-degrading bacteria belonged to Enterobacteriaceae and Bacillaceae families. Several isolates recovered from separate termite Microcerotermes diversus samples closely clustered in phylogenetic trees indicating high similarity and the abundance of particular cellulolytic strains. Bacillus B5B and Acinetobacter L9B hydrolyzed cellulose faster than the other isolates (with CMCase activity of 1.47 U/mL and 1.22 U/mL, respectively). The stability of CMCase produced by Bacillus B5B over a broad range of pH and high temperature indicated that the enzyme may be of great commercial value.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
羧甲基纤维素钠, average Mw ~90,000
Sigma-Aldrich
纤维素, microcrystalline, powder
Sigma-Aldrich
羧甲基纤维素 钠盐, low viscosity
Sigma-Aldrich
纤维素, microcrystalline, powder, 20 μm
Sigma-Aldrich
纤维素, fibers, (medium)
Sigma-Aldrich
羧甲基纤维素 钠盐, Medium viscosity
Supelco
Avicel® PH-101, ~50 μm particle size
Sigma-Aldrich
刚果红, Dye content ≥35 %
Sigma-Aldrich
纤维素酶 来源于里氏木霉, aqueous solution, ≥700 units/g
Sigma-Aldrich
纤维素酶 来源于黑曲霉, powder, ≥0.3 units/mg solid
Sigma-Aldrich
羧甲基纤维素 钠盐, High viscosity
Sigma-Aldrich
Sigmacell 纤维素, Type 20, 20 μm
Sigma-Aldrich
纤维素酶 来源于木霉 属, BioReagent, suitable for plant cell culture, 3-10 units/mg solid
Sigma-Aldrich
纤维素酶 来源于曲霉 属, aqueous solution
Sigma-Aldrich
α纤维素, powder
Sigma-Aldrich
羧甲基纤维素钠, average Mw ~700,000
Sigma-Aldrich
Avicel® PH-101, tested according to Ph. Eur.
Sigma-Aldrich
羧甲纤维素 钠, meets USP testing specifications, Medium viscosity
Sigma-Aldrich
羧甲基纤维素钠, average Mw ~250,000, degree of substitution 0.7
Sigma-Aldrich
刚果红, certified by the Biological Stain Commission, BioXtra
Sigma-Aldrich
羧甲基纤维素钠, average Mw ~250,000, degree of substitution 1.2
Sigma-Aldrich
羧甲基纤维素钠, average Mw ~250,000, degree of substitution 0.9
Sigma-Aldrich
纤维素酶 来源于里氏木霉 ATCC 26921, lyophilized powder, ≥1 unit/mg solid
Sigma-Aldrich
纤维素酶 来源于木霉 属, powder, ≥5,000 units/g solid
Sigma-Aldrich
纤维素, colloidal, microcrystalline
Sigma-Aldrich
羧甲基纤维素 钠盐, medium viscosity
Sigma-Aldrich
Sigmacell 纤维素, Type 101, Highly purified, fibers
Sigma-Aldrich
羧甲基纤维素 钠盐, ultra high viscosity, highly purified
Sigma-Aldrich
纤维素酶 来源于黑曲霉, powder, off-white, ~0.8 U/mg
Supelco
纤维素, powder, for column chromatography