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Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation.

Bioprocess and biosystems engineering (2011-07-23)
Luciane Batistella, Mara K Ustra, Aline Richetti, Sibele B C Pergher, Helen Treichel, J V Oliveira, Lindomar Lerin, Débora de Oliveira
ABSTRACT

Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure, the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,2,4-三甲基戊烷, anhydrous, 99.8%
Sigma-Aldrich
2,2,4-三甲基戊烷, ACS reagent, ≥99.0%
Sigma-Aldrich
2,2,4-三甲基戊烷, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.5%
Sigma-Aldrich
2,2,4-三甲基戊烷, suitable for HPLC, ≥99%
Sigma-Aldrich
2,2,4-三甲基戊烷, puriss. p.a., ≥99.5% (GC)
Supelco
2,2,4-三甲基戊烷, analytical standard
Sigma-Aldrich
2,2,4-三甲基戊烷, ReagentPlus®, ≥99%
Sigma-Aldrich
2,2,4-三甲基戊烷, puriss. p.a., ACS reagent, ≥99.5% (GC)
Supelco
密度标准品 692kg/m3, H&D Fitzgerald Ltd. Quality