Merck
  • Home
  • Search Results
  • Microchip UV absorbance detection applied to isoelectric focusing of proteins.

Microchip UV absorbance detection applied to isoelectric focusing of proteins.

Methods in molecular biology (Clifton, N.J.) (2013-01-19)
Junjie Ou, Carolyn L Ren
ABSTRACT

Isoelectric focusing (IEF) is considered as an attractive separation technique for biologically amphoteric compounds (e.g., proteins and peptides) based on their isoelectric point (pI). With the advancement in micromachining technology, microchip format IEF has attracted significant attention. Both single-point and whole column imaging detection (WCID) methods have been employed for analyzing the separation performance in a microchip. WCID is more favorable than single-point detection because the latter requires the focused bands to be mobilized and thus adds more complexity to the design and operation of such microchips. Fluorescence- and UV absorbance-based WCID have been successfully adapted in glass and PDMS microchips. We have developed polydimethylsiloxane (PDMS) microchips for IEF applications where UV-WCID is employed for evaluating the separation performance. The chips are designed for use in the iCE280 analyzer (Convergent Bioscience Inc., Toronto), for capillary-based IEF where UV-WCID is employed for analyzing the separation performance. Three kinds of microchips that have been successfully developed using standard soft lithography technology are described in detail.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
硅油, for oil baths (from −50°C to +200°C)
Sigma-Aldrich
硅油, viscosity 5 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 10 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 20 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 1,000 cSt (25 °C)
Sigma-Aldrich
硅油, Dow Corning 200® fluid, viscosity 60,000 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 100 cSt (25 °C)
硅油, for melting point and boiling point apparatuses
Sigma-Aldrich
硅油, viscosity 350 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 10,000 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 50 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 500 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 100,000 cSt (25 °C)
Sigma-Aldrich
硅油, viscosity 30,000 cSt (25 °C)