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A pathology-MRI study of the short-T2 component in formalin-fixed multiple sclerosis brain.

Neurology (2000-11-30)
G R Moore, E Leung, A L MacKay, I M Vavasour, K P Whittall, K S Cover, D K Li, S A Hashimoto, J Oger, T J Sprinkle, D W Paty
摘要

To determine the pathologic basis of areas not exhibiting signal of the short-T2 component of the T2 relaxation distribution in MS, as studied in formalin-fixed brain. A myelin-specific MRI signal would be of great importance in assessing demyelination in patients with MS. Evidence indicates that the short-T2 (10 to 50 millisecond) component of the T2 relaxation distribution originates from water in myelin sheaths. The authors present two cases of MS in which the anatomic distribution of the short-T2 component was correlated with the pathologic findings in postmortem formalin-fixed brain. One half of the formalin-fixed brain was suspended in a gelatin-albumin mixture cross-linked with glutaraldehyde, and scanned with a 32-echo MRI sequence. The brain was then cut along the center of the 5-mm slices scanned, photographed, dehydrated, and embedded in paraffin. Paraffin sections, stained with Luxol fast blue and immunocytochemically for 2',3'-cyclic nucleotide 3'-phosphohydrolase for myelin and by the Bielschowsky technique for axons, were compared with the distribution of the amplitude of the short-T2 component of the comparable image slices. The anatomic distribution of the short-T2 component signal corresponded to the myelin distribution. Chronic, silent MS plaques with myelin loss correlated with areas of absence of short-T2 signal. The numbers of axons within lesions were reduced, but many surviving axons were also seen in these areas of complete loss of myelin. In formalin-fixed MS brains the short-T2 component of the T2 relaxation distribution corresponds to the anatomic distribution of myelin. Chronic, silent demyelinated MS plaques show absence of the short-T2 component signal. These results support the hypothesis that the short-T2 component originates from water related to myelin.-1510

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甲醛 溶液, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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甲醛 溶液, for molecular biology, 36.5-38% in H2O
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甲醛 溶液, for molecular biology, BioReagent, ≥36.0% in H2O (T)
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甲醛 溶液, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
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甲醛 溶液, meets analytical specification of USP, ≥34.5 wt. %
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甲醛 溶液, stabilized with methanol, ~37 wt. % in H2O, certified reference material
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甲醛 溶液, tested according to Ph. Eur.