Merck
  • 主页
  • 查找结果
  • Differential acetylcholine and choline concentrations in the cerebrospinal fluid of patients with Alzheimer's disease and vascular dementia.

Differential acetylcholine and choline concentrations in the cerebrospinal fluid of patients with Alzheimer's disease and vascular dementia.

Chinese medical journal (2004-09-14)
Jian-ping Jia, Jian-min Jia, Wei-dong Zhou, Min Xu, Chang-biao Chu, Xin Yan, Yong-xin Sun
摘要

An important aspect of Alzheimer's disease (AD) is loss or impairment of cholinergic neurons. It is controversial whether there is a similar cholinergic impairment and cerebral deficit of acetylcholine (ACh) in the case of vascular dementia (VD). The purpose of this study was to explore the levels of ACh and choline (Ch) in the cerebrospinal fluid (CSF) of patients with AD and VD, and their possible relationship with cognitive impairment. Twenty-two AD patients, twenty-two VD patients, and twenty normal controls were recruited and scored with a Mini-Mental State Examination (MMSE). CSF concentrations of ACh and Ch were measured using high-performance liquid chromatography with an electrochemical detector (HPLC-ECD) and the results were then compared to cognitive status. ACh concentrations in CSF of AD patients [(10.7 +/- 5.1) nmol/L] and VD patients [(16.8 +/- 7.4) nmol/L] were both significantly lower than in controls [(34.5 +/- 9.0) nmol/L, t = 10.67, P < 0.001; t = 6.91, P < 0.001]. Both results correlated positively with MMSE scores (rs = 0.88 and rs = 0.85, respectively, P < 0.01). The CSF concentration of Ch was significantly higher in VD patients [(887.4 +/- 187.4) nmol/L] compared to AD patients [(627.6 +/- 145.1) nmol/L, t = 6.4, P < 0.001] and controls [(716.0 +/- 159.4) nmol/L, t = 4.2, P = 0.002]. CSF Ch concentration showed no difference between AD patients and normal controls, nor did it correlate with MMSE score in any of the three groups. The positive correlation between ACh deficit and cognitive impairment suggests that ACh is an important neurotransmitter for memory. The similar decrease in ACh concentration in AD and VD patients may imply a similar pathogenesis for the process of cognitive impairment involved in these two disorders. The elevated CSF levels of Ch in VD patients compared to AD patients may be useful diagnostically. Cholinesterase inhibitors may be helpful not only for AD patients, but also for VD patients.

材料
货号
品牌
产品描述

Sigma-Aldrich
甘氨酸, suitable for electrophoresis, ≥99%
Sigma-Aldrich
甘氨酸, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
L -谷氨酰胺 溶液, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
D -山梨醇, ≥98%
Sigma-Aldrich
D -甘露醇, ≥98%
Sigma-Aldrich
L-谷氨酰胺, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
Sigma-Aldrich
L-脯氨酸, from non-animal source, meets EP, USP testing specifications, suitable for cell culture
Sigma-Aldrich
肌醇 -肌醇, ≥99%
Sigma-Aldrich
L-谷氨酸, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
L-脯氨酸, ReagentPlus®, ≥99% (HPLC)
SAFC
L-谷氨酰胺溶液 200mM, 29.23 mg/mL in saline, solution, suitable for cell culture
Sigma-Aldrich
L -赖氨酸, ≥98% (TLC)
Sigma-Aldrich
甘氨酸, BioUltra, for molecular biology, ≥99.0% (NT)
Sigma-Aldrich
L-亮氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-异亮氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
肌醇 -肌醇, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
L-异亮氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-酪氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
锌, dust, <10 μm, ≥98%
Sigma-Aldrich
L-亮氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-谷氨酰胺, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
L-苯基丙氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
SAFC
L-谷氨酰胺
Supelco
山梨醇, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-丝氨酸, ReagentPlus®, ≥99% (HPLC)
Supelco
甘露醇, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
左旋多巴, ≥98% (TLC)
Sigma-Aldrich
γ-氨基丁酸, ≥99%
Sigma-Aldrich
锌, granular, 20-30 mesh, ACS reagent, ≥99.8%
Sigma-Aldrich
L-苯基丙氨酸, reagent grade, ≥98%