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  • Epithelial bridges maintain tissue integrity during collective cell migration.

Epithelial bridges maintain tissue integrity during collective cell migration.

Nature materials (2013-12-03)
Sri Ram Krishna Vedula, Hiroaki Hirata, Mui Hoon Nai, Agustí Brugués, Yusuke Toyama, Xavier Trepat, Chwee Teck Lim, Benoit Ladoux
ABSTRACT

The ability of skin to act as a barrier is primarily determined by the efficiency of skin cells to maintain and restore its continuity and integrity. In fact, during wound healing keratinocytes migrate collectively to maintain their cohesion despite heterogeneities in the extracellular matrix. Here, we show that monolayers of human keratinocytes migrating along functionalized micropatterned surfaces comprising alternating strips of extracellular matrix (fibronectin) and non-adherent polymer form suspended multicellular bridges over the non-adherent areas. The bridges are held together by intercellular adhesion and are subjected to considerable tension, as indicated by the presence of prominent actin bundles. We also show that a model based on force propagation through an elastic material reproduces the main features of bridge maintenance and tension distribution. Our findings suggest that multicellular bridges maintain tissue integrity during wound healing when cell-substrate interactions are weak and may prove helpful in the design of artificial scaffolds for skin regeneration.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Fibrinogen 来源于牛血浆, Type I-S, 65-85% protein (≥75% of protein is clottable)
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硅油, viscosity 20 cSt (25 °C)
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硅油, viscosity 1,000 cSt (25 °C)
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纤维蛋白原 来源于人类血浆, 35-65% protein (≥90% of protein is clottable).
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硅油, Dow Corning 200® fluid, viscosity 60,000 cSt (25 °C)
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硅油, viscosity 100 cSt (25 °C)
硅油, for melting point and boiling point apparatuses
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硅油, viscosity 350 cSt (25 °C)
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硅油, viscosity 10,000 cSt (25 °C)
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硅油, viscosity 50 cSt (25 °C)
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硅油, viscosity 500 cSt (25 °C)
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硅油, viscosity 100,000 cSt (25 °C)
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硅油, viscosity 30,000 cSt (25 °C)
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纤维蛋白原 来源于大鼠血浆, 60-80% protein (≥60% of protein is clottable)