Merck
  • 主页
  • 查找结果
  • Involvement of alanine racemase in germination of Bacillus cereus spores lacking an intact exosporium.

Involvement of alanine racemase in germination of Bacillus cereus spores lacking an intact exosporium.

Archives of microbiology (2013-12-19)
Elena Venir, Manuela Del Torre, Vincenzo Cunsolo, Rosaria Saletti, Rita Musetti, Mara Lucia Stecchini
摘要

The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-alanine. Proteomic analysis of the alkaline extracts of the spore proteins, which include exosporium and coat proteins, confirmed that Alr was present in the B. cereus DSA 1 spores and matched to that encoded by B. cereus ATCC 14579, whose spore germination was strongly affected by the block of conversion of L- to D-alanine. Unlike ATCC 14579 spores, L-alanine germination of B. cereus DSA 1 spores was not affected by the preincubation with DCS, suggesting a lack of restriction in the reactant accessibility.

材料
货号
品牌
产品描述

Sigma-Aldrich
L-丙氨酸, ≥98% (TLC)
Sigma-Aldrich
D-环丝氨酸
Sigma-Aldrich
L-丙氨酸, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-丙氨酸, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
D-环丝氨酸
Sigma-Aldrich
L-丙氨酸, ≥99%
Supelco
L-丙氨酸, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-丙氨酸, ≥99.0% (NT)
丙氨酸, European Pharmacopoeia (EP) Reference Standard
Supelco
L-丙氨酸, certified reference material, TraceCERT®
Sigma-Aldrich
D-环丝氨酸, synthetic
Sigma-Aldrich
L-Alanine-12C3, 99.9 atom % 12C