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  • In vitro antitumor activity of parent and nano-encapsulated mono cobalt-substituted Keggin polyoxotungstate and its ctDNA binding properties.

In vitro antitumor activity of parent and nano-encapsulated mono cobalt-substituted Keggin polyoxotungstate and its ctDNA binding properties.

Chemico-biological interactions (2014-03-13)
S Dianat, A K Bordbar, S Tangestaninejad, B Yadollahi, S H Zarkesh-Esfahani, P Habibi
摘要

The parent and nanosized starch, and lipid encapsulated K6[SiW11O39Co(H2O)]·nH2O (abbreviated as SEP, LEP and SiW11Co, respectively), as potent antitumor candidates, were synthesized and characterized by FT-IR spectroscopy, ICP, TG analysis, SEM and TEM images. The results show that the SiW11Co retains its parent structure after encapsulation by starch and lipid nanoparticles. Antitumor activity tests of SiW11Co and its encapsulated forms were carried out on two types of human cancer cells, MCF-7 and HEK-293 by MTT method. The encapsulated forms exhibited the higher antitumor activity compared to the parent SiW11Co. However, this observed enhancement for the lipid encapsulated form is more than the starch counterpart, which can be related to its smaller size. These results showed that these compounds can be novel antitumor candidates. The calf thymus DNA (abbreviated as ctDNA) binding ability of SiW11Co was also investigated, using UV-Vis absorption spectroscopy, fluorescence quenching and fluorescence Scatchard plots. Absorption spectra tracing reveal 10% hyperchromism for SiW11Co. The values of 1.8×10(4) and 1.2×10(4)M(-1) were obtained for association binding constant of SiW11Co to ctDNA at R⩾1 and R<1, respectively (R is defined as the mole ratio of SiW11Co to ctDNA). It was shown that the interaction of SiW11Co with ctDNA depended on the R values. The obtained results of absorption titration rejected the intercalating binding mode and suggest the groove or outside stacking binding for SiW11Co. These results were authenticated by fluorescence quenching experiments and scatchard plots.

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Sigma-Aldrich
淀粉 来源于马铃薯, Soluble
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淀粉 来源于玉米
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淀粉, puriss. p.a., from potato, reag. ISO, reag. Ph. Eur., soluble
Sigma-Aldrich
脱氧核糖核酸 来源于小牛胸腺, Type XV, Activated, lyophilized powder
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淀粉, from potato, tested according to Ph. Eur.
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淀粉 来源于玉米, practical grade
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淀粉 来源于大米
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淀粉 来源于马铃薯, Powder
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淀粉 来源于小麦, Unmodified
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钴, powder, <150 μm, ≥99.9% trace metals basis
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钴, powder, 2 μm particle size, 99.8% trace metals basis
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脱氧核糖核酸 来源于小牛胸腺, Genomic, unsheared
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淀粉, from potato, acc. to Zulkowsky (starch, treated with glycerol at 190°C)
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脱氧核糖核酸,单链 来源于小牛胸腺, lyophilized powder
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淀粉 来源于玉米, Unmodified waxy corn starch of essentially pure amylopectin; contains only trace amounts of amylose.
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淀粉 来源于马铃薯, suitable for electrophoresis
Supelco
淀粉 来源于玉米, analytical standard, analytical standard for Starch Assay Kits SA-20 and STA-20
Sigma-Aldrich
钴, Carbon coated magnetic, nanopowder, <50 nm particle size (TEM), ≥99%
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钴, granular, 99.99% trace metals basis
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钴, foil, thickness 0.1 mm, 99.95% trace metals basis
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钴, pieces, 99.5% trace metals basis
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钴, wire, diam. 1.0 mm, 99.995% trace metals basis
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钴, foil, thickness 0.25 mm, ≥99.99% trace metals basis
钴, wire reel, 0.1m, diameter 0.5mm, hard, 99.99+%
钴, foil, 25mm disks, thickness 0.05mm, annealed, 99.9%
钴, foil, 25mm disks, thickness 0.05mm, as rolled, 99.9%
钴, foil, 25mm disks, thickness 0.125mm, annealed, 99.9%
钴, foil, 25mm disks, thickness 0.25mm, as rolled, 99.9%
钴, foil, 25mm disks, thickness 0.25mm, as rolled, 99.99+%
钴, foil, 4mm disks, thickness 0.003mm, 99.9%