Merck
  • 主页
  • 查找结果
  • An overview of the visualisation and quantitation of low and high MW DNA adducts using the trapped in agarose DNA immunostaining (TARDIS) assay.

An overview of the visualisation and quantitation of low and high MW DNA adducts using the trapped in agarose DNA immunostaining (TARDIS) assay.

Mutagenesis (2010-11-12)
Ian G Cowell, Michael J Tilby, Caroline A Austin
摘要

The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and cisplatin DNA adducts at the single-cell level and has since been adapted to quantify topoisomerase-DNA complexes. The method relies on salt-detergent extraction of agarose-embedded cells. Genomic DNA and any covalently attached molecules remain in place in the agarose, while other cellular constituents are removed. Drug-DNA or topoisomerase-DNA complexes are then detected and quantified by sensitive immunofluorescence using adduct-specific antibodies. Here, we give a perspective of the TARDIS assay including a comparison with other methods for quantifying topoisomerase-DNA covalent complexes and provide technical details required to set up and perform the assay.

材料
货号
品牌
产品描述

Sigma-Aldrich
琼脂糖, BioReagent, for molecular biology, low EEO
Sigma-Aldrich
琼脂糖, Type I, low EEO
Sigma-Aldrich
琼脂糖, for molecular biology
Sigma-Aldrich
琼脂糖, Low EEO
Sigma-Aldrich
琼脂糖, Ultra-low Gelling Temperature, molecular biology grade
Sigma-Aldrich
琼脂糖, Medium EEO, for molecular biology
Sigma-Aldrich
Sepharose® 4B, 45-165 μm bead diameter
Sigma-Aldrich
琼脂糖, Type I-A, low EEO
Sigma-Aldrich
琼脂糖, Ultra-low Gelling Temperature
Sigma-Aldrich
琼脂糖, Low EEO, for Immunoelectrophoresis
Sigma-Aldrich
琼脂糖, Type II-A, Medium EEO
Sigma-Aldrich
琼脂糖, High Gelling Temperature
Sigma-Aldrich
琼脂糖, High EEO, for molecular biology
Sigma-Aldrich
Sepharose® 6B, 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)
Sigma-Aldrich
琼脂糖, Wide range, for molecular biology
Sigma-Aldrich
琼脂糖, High EEO
Sigma-Aldrich
琼脂糖, For pulsed field electrophoresis running gel
Sigma-Aldrich
琼脂糖, BioReagent, for molecular biology, Wide range/Standard 3:1
Sigma-Aldrich
琼脂糖, Special High EEO
Sigma-Aldrich
琼脂糖, Type IV, Special High EEO
Sigma-Aldrich
琼脂糖, Vetec, reagent grade