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  • Effects of chemical and thermal stress on acetylcholinesterase activity in the brain of the bank vole, Myodes glareolus.

Effects of chemical and thermal stress on acetylcholinesterase activity in the brain of the bank vole, Myodes glareolus.

Ecotoxicology and environmental safety (2014-05-24)
Renata Świergosz-Kowalewska, Patrycja Molenda, Anna Halota
摘要

One of the most important issues in ecotoxicology is better understanding the effects of interactions between chemical pollutants and physical environmental factors on animals. To fill this knowledge gap, changes in the activity of acetylcholinesterase (AChE) in the brain samples of bank voles Myodes (Clethrionomys) glareolus due to temperature effects, and two chemical stressors were studied in a full factorial laboratory experiment (27 treatments). The experiment was divided into three phases: acclimatisation (3 days), intoxication (42 days) and elimination (21 days). During the intoxication phase, animals were orally exposed to different concentrations of either nickel (0, 300 or 800 mg Ni/kg food), chlorpyrifos (CPF) (0, 50 or 350 mg CPF/kg food) or a mixture of both chemicals. During the acclimatisation and elimination phases, the bank voles were given uncontaminated food. The experiment was conducted at three different temperatures (10, 20 or 30 °C), and a 12 h:12 h light:dark regime. The animals were sacrificed at 0, 5, 10, 20, 42, 49 and 63 days after the beginning of the intoxication, and brain samples were obtained for chemical analysis. The nickel accumulation in the brain depended on the level of nickel exposure and on interactions between the temperature and other factors. Nickel exhibited no effect on AChE activity. In contrast, AChE was drastically inhibited by chlorpyrifos and low temperature, but interactions between all factors significantly influenced the enzyme activity during the elimination phase of the experiment. High mortality was observed in the groups exposed to high concentrations of nickel and chlorpyrifos.

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Supelco
毒死蜱, PESTANAL®, analytical standard
Supelco
毒死蜱, certified reference material, TraceCERT®
Sigma-Aldrich
镍, foil, thickness 0.125 mm, ≥99.9%
Sigma-Aldrich
镍, foil, thickness 0.5 mm, 99.98% trace metals basis
Sigma-Aldrich
镍, wire, diam. 0.25 mm, ≥99.9%
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毒死蜱 溶液, 100 μg/mL in acetonitrile, PESTANAL®, analytical standard
Sigma-Aldrich
镍, foil, thickness 0.1 mm, 99.98% trace metals basis
Sigma-Aldrich
镍, wire, diam. 0.5 mm, ≥99.9% trace metals basis
Sigma-Aldrich
镍, wire, diam. 0.5 mm, ≥99.99% trace metals basis
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镍, rod, diam. 6.35 mm, ≥99.99% trace metals basis
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镍, foil, thickness 0.25 mm, 99.995% trace metals basis
镍, mesh, 100x250mm, nominal aperture 0.73mm, wire diameter 0.25mm, 26x26 wires/inch, open area 55%, plain weave mesh, 99%
镍, wire reel, 20m, diameter 0.025mm, as drawn, 99.98%
镍, wire reel, 10m, diameter 0.01mm, as drawn, 99.98%
镍, rod, 100mm, diameter 16mm, 99.99+%
镍, foil, not light tested, 100x100mm, thickness 0.01mm, 99.95%
镍, wire reel, 20m, diameter 0.125mm, hard, 99.98%
镍, rod, 100mm, diameter 3.0mm, 99%
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镍, tube, 1000mm, outside diameter 6.5mm, inside diameter 5.5mm, wall thickness 0.5mm, hard, 99.5%
镍, foil, 50mm disks, thickness 0.05mm, annealed, 99%
镍, wire reel, 20m, diameter 0.5mm, as drawn, 99%
镍, foil, 4mm disks, thickness 0.075mm, annealed, 99%
镍, foil, 4mm disks, thickness 0.075mm, as rolled, 99.98%
镍, foil, 4mm disks, thickness 0.125mm, annealed, 99%
镍, foil, 4mm disks, thickness 0.125mm, annealed, 99.98%
镍, foil, 4mm disks, thickness 0.125mm, as rolled, 100%
镍, foil, 4mm disks, thickness 0.125mm, as rolled, 99.99+%
镍, foil, 4mm disks, thickness 0.15mm, annealed, 99%