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  • Contaminant-specific targeting of olfactory sensory neuron classes: connecting neuron class impairment with behavioural deficits.

Contaminant-specific targeting of olfactory sensory neuron classes: connecting neuron class impairment with behavioural deficits.

Chemosphere (2014-03-19)
William A Dew, Ali Azizishirazi, Greg G Pyle
摘要

The olfactory system of fish comprises several classes of olfactory sensory neurons (OSNs). The odourants L-alanine and taurocholic acid (TCA) specifically activate microvillous or ciliated OSNs, respectively, in fish. We recorded electro-olfactograms (EOG) in fathead minnows (Pimephales promelas; a laboratory-reared model species) and wild yellow perch (Perca flavescens) whose olfactory chambers were perfused with either L-alanine or TCA to determine if OSN classes were differentially vulnerable to contaminants, in this case copper or nickel. Results were consistent in both species and demonstrated that nickel targeted and impaired microvillous OSN function, while copper targeted and impaired ciliated OSN function. This result suggests that contaminant-specific effects observed in model laboratory species extrapolate to wild fish populations. Moreover, fathead minnows exposed to copper failed to perceive a conspecific alarm cue in a choice maze, whereas those exposed to nickel could respond to the same conspecific cue. These results demonstrate that fathead minnows perceive conspecific, damage-released alarm cue by ciliated, but not microvillous, OSNs. Fish living in copper-contaminated environments may be more vulnerable to predation than those in clean lakes owing to targeted effects on ciliated OSNs.

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Sigma-Aldrich
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镍, tube, 500mm, outside diameter 1.8mm, inside diameter 1.6mm, wall thickness 0.1mm, hard, 99.5%
镍, wire reel, 20m, diameter 0.025mm, as drawn, 99.98%
镍, wire reel, 1m, diameter 1.0mm, as drawn, 99%
镍, mesh, 100x250mm, nominal aperture 0.73mm, wire diameter 0.25mm, 26x26 wires/inch, open area 55%, plain weave mesh, 99%
镍, sphere, 100pcs, diameter 1.0mm, precision sphere grade 200, 99%
镍, foil, 50x50mm, thickness 0.30mm, as rolled, 99.98%
镍, wire reel, 5m, diameter 1.0mm, hard, 99.98%
镍, wire reel, 1m, diameter 0.75mm, as drawn, 99%
镍, rod, 100mm, diameter 12.7mm, 99.98%
镍, sphere, 200pcs, diameter 3.0mm, precision sphere grade 200, 99%
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