Merck
  • Home
  • Search Results
  • Evaluation of the leucine incorporation technique for detection of pollution-induced community tolerance to copper in a long-term agricultural field trial with urban waste fertilizers.

Evaluation of the leucine incorporation technique for detection of pollution-induced community tolerance to copper in a long-term agricultural field trial with urban waste fertilizers.

Environmental pollution (Barking, Essex : 1987) (2014-08-06)
Jonas Duus Stevens Lekfeldt, Jakob Magid, Peter E Holm, Ole Nybroe, Kristian Koefoed Brandt
ABSTRACT

Copper (Cu) is known to accumulate in agricultural soils receiving urban waste products as fertilizers. We here report the use of the leucine incorporation technique to determine pollution-induced community tolerance (Leu-PICT) to Cu in a long-term agricultural field trial. A significantly increased bacterial community tolerance to Cu was observed for soils amended with organic waste fertilizers and was positively correlated with total soil Cu. However, metal speciation and whole-cell bacterial biosensor analysis demonstrated that the observed PICT responses could be explained entirely by Cu speciation and bioavailability artifacts during Leu-PICT detection. Hence, the agricultural application of urban wastes (sewage sludge or composted municipal waste) simulating more than 100 years of use did not result in sufficient accumulation of Cu to select for Cu resistance. Our findings also have implications for previously published PICT field studies and demonstrate that stringent PICT detection criteria are needed for field identification of specific toxicants.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-亮氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-亮氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
铜, powder, <425 μm, 99.5% trace metals basis
Sigma-Aldrich
铜, wire, diam. 1.0 mm, ≥99.9%
Sigma-Aldrich
铜, foil, thickness 0.25 mm, 99.98% trace metals basis
Sigma-Aldrich
铜, powder, 99.999% trace metals basis
Sigma-Aldrich
铜, ACS reagent, granular, 10-40 mesh, ≥99.90%
Supelco
L-亮氨酸, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
铜, nanopowder, 60-80 nm particle size (SAXS), ≥99.5% trace metals basis
Sigma-Aldrich
L-亮氨酸, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
铜, foil, thickness 0.025 mm, 99.98% trace metals basis
Sigma-Aldrich
铜, foil, ≥99.8% (complexometric)
Sigma-Aldrich
铜, powder, <75 μm, 99%
Sigma-Aldrich
铜, powder (spheroidal), 10-25 μm, 98%
Sigma-Aldrich
L-亮氨酸, 99%, FG
Sigma-Aldrich
铜, foil, thickness 0.5 mm, 99.98% trace metals basis
Sigma-Aldrich
铜, foil, thickness 1.0 mm, 99.999% trace metals basis
Sigma-Aldrich
铜, bars (random sizes), ≥99.999% trace metals basis
Sigma-Aldrich
铜, powder (dendritic), <45 μm, 99.7% trace metals basis
Sigma-Aldrich
铜, turnings, purum p.a., ≥99.0%
Sigma-Aldrich
铜, wire, diam. 0.64 mm, 99.995% trace metals basis
Supelco
L-亮氨酸, certified reference material, TraceCERT®
Sigma-Aldrich
铜, wire, diam. 0.25 mm, 99.999% trace metals basis
Sigma-Aldrich
铜, beads, 2-8 mm, ≥99.99% trace metals basis
亮氨酸, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
铜, shot, −3-+14 mesh, 99%
Sigma-Aldrich
铜, wire, diam. 2.0 mm, 99.999% trace metals basis
Sigma-Aldrich
铜, beads, 2-8 mm, 99.9995% trace metals basis
铜, mesh, 130x130mm, nominal aperture 0.2mm, thickness 0.004mm, wire diameter 0.0125mm, 118 wires/inch, open area 88%, electro-formed mesh
铜, mesh, 100x100mm, nominal aperture 0.14mm, thickness 0.25mm, wire diameter 0.115mm, 100x100 wires/inch, open area 30.3%, plain weave mesh