Merck
  • Home
  • Search Results
  • Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique.

Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique.

Journal of clinical microbiology (1979-12-01)
K H Young, S L Bullock, D M Melvin, C L Spruill
PMID574877
ABSTRACT

Ethyl acetate appears to be a satisfactory subsitute solvent for diethyl ether in the Formalin-ether sedimentation technique. In comparative studies, concentration of organisms with ethyl acetate was equal to or greater than that with diethyl ether. No distortion or alteration of morphology was observed with eigher solvent, and preparations were comparable in appearance and ease of examination. In addition, ethyl acetate is less flammable and less hazardous to use than diethyl ether.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
二乙醚, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
Sigma-Aldrich
乙酸乙酯, anhydrous, 99.8%
Sigma-Aldrich
二乙醚, contains BHT as inhibitor, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
Sigma-Aldrich
乙酸乙酯, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99.5% (GC)
Sigma-Aldrich
乙酸乙酯, suitable for HPLC, ≥99.8%
Sigma-Aldrich
乙酸乙酯, ≥99%, FCC, FG
Sigma-Aldrich
乙酸乙酯, ReagentPlus®, ≥99.8%
Sigma-Aldrich
乙酸乙酯, ACS reagent, ≥99.5%
Sigma-Aldrich
二乙醚, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)
Sigma-Aldrich
乙酸乙酯, biotech. grade, ≥99.8%