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  • Transcriptional and biochemical effects of cadmium and manganese on the defense system of Octopus vulgaris paralarvae.

Transcriptional and biochemical effects of cadmium and manganese on the defense system of Octopus vulgaris paralarvae.

BioMed research international (2015-02-24)
Aldo Nicosia, Monica Salamone, Salvatore Mazzola, Angela Cuttitta
摘要

Due to anthropogenic activities the relative concentrations of cadmium and manganese have increased in the marine environment. Cephalopods are able to accumulate such metals and, as inhabitant of coastal waters, Octopus vulgaris is continuously exposed to anthropogenic activities. Since no study is available on the effects of heavy metals at molecular level in developing octopuses, herein we exposed 1-day-old paralarvae for 24 h to 10, 100, and 1000 μg/L of CdCl2 or MnCl2. Cd exerted a concentration-dependent inhibition of survival and a reduction in growth rate was shown while Mn exposure did not affect the survival rate even at the highest concentrations. Gene expression profiles of hsp70, sod, cat, and gst genes were analyzed by quantitative real-time PCR and defined patterns of transcription were observed. Moreover posttranscriptional analyses were also performed suggesting the impairment of metabolic functions, under strong oxidative conditions (as occurred in paralarvae exposed to Cd) or the complete detoxification events (as occurred in paralarvae exposed to Mn).

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Sigma-Aldrich
单克隆抗 β-肌动蛋白抗体 小鼠抗, clone AC-74, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
锰, powder, ≥99.9% trace metals basis
Sigma-Aldrich
锰, powder, −325 mesh, ≥99% trace metals basis
Sigma-Aldrich
单克隆抗-热休克蛋白 70 小鼠抗, clone BRM-22, ascites fluid
Sigma-Aldrich
锰, chips, thickness <2.0 mm, 99%
Sigma-Aldrich
抗 热休克蛋白 70 (HSP70) 抗体,小鼠单克隆, clone BRM-22, purified from hybridoma cell culture
锰, rod, 10mm, diameter 4.0mm, cast, 99.5%
锰, foil, 25x25mm, thickness 2mm, hot-pressed, 99.95%
锰, foil, 50x50mm, thickness 2mm, hot-pressed, 99.95%
锰, foil, not light tested, 100x100mm, thickness 0.0125mm, permanent polyester support, 98.7%
锰, foil, not light tested, 100x100mm, thickness 0.01mm, permanent polyester support, 98.7%
锰, foil, not light tested, 100x100mm, thickness 0.025mm, permanent polyester support, 98.7%
锰, foil, not light tested, 100x100mm, thickness 0.02mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.001mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.0025mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.002mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.003mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.004mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.005mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.006mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.007mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.008mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.009mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.0125mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.01mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.025mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.05mm, permanent polyester support, 98.7%
锰, foil, not light tested, 50x50mm, thickness 0.001mm, permanent polyester support, 98.7%
锰, foil, not light tested, 50x50mm, thickness 0.0025mm, permanent polyester support, 98.7%
锰, foil, not light tested, 50x50mm, thickness 0.003mm, permanent polyester support, 98.7%