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  • Differential effects of 670 and 830 nm red near infrared irradiation therapy: a comparative study of optic nerve injury, retinal degeneration, traumatic brain and spinal cord injury.

Differential effects of 670 and 830 nm red near infrared irradiation therapy: a comparative study of optic nerve injury, retinal degeneration, traumatic brain and spinal cord injury.

PloS one (2014-08-12)
Marcus K Giacci, Lachlan Wheeler, Sarah Lovett, Emma Dishington, Bernadette Majda, Carole A Bartlett, Emma Thornton, Elizabeth Harford-Wright, Anna Leonard, Robert Vink, Alan R Harvey, Jan Provis, Sarah A Dunlop, Nathan S Hart, Stuart Hodgetts, Riccardo Natoli, Corinna Van Den Heuvel, Melinda Fitzgerald
摘要

Red/near-infrared irradiation therapy (R/NIR-IT) delivered by laser or light-emitting diode (LED) has improved functional outcomes in a range of CNS injuries. However, translation of R/NIR-IT to the clinic for treatment of neurotrauma has been hampered by lack of comparative information regarding the degree of penetration of the delivered irradiation to the injury site and the optimal treatment parameters for different CNS injuries. We compared the treatment efficacy of R/NIR-IT at 670 nm and 830 nm, provided by narrow-band LED arrays adjusted to produce equal irradiance, in four in vivo rat models of CNS injury: partial optic nerve transection, light-induced retinal degeneration, traumatic brain injury (TBI) and spinal cord injury (SCI). The number of photons of 670 nm or 830 nm light reaching the SCI injury site was 6.6% and 11.3% of emitted light respectively. Treatment of rats with 670 nm R/NIR-IT following partial optic nerve transection significantly increased the number of visual responses at 7 days after injury (P ≤ 0.05); 830 nm R/NIR-IT was partially effective. 670 nm R/NIR-IT also significantly reduced reactive species and both 670 nm and 830 nm R/NIR-IT reduced hydroxynonenal immunoreactivity (P ≤ 0.05) in this model. Pre-treatment of light-induced retinal degeneration with 670 nm R/NIR-IT significantly reduced the number of Tunel+ cells and 8-hydroxyguanosine immunoreactivity (P ≤ 0.05); outcomes in 830 nm R/NIR-IT treated animals were not significantly different to controls. Treatment of fluid-percussion TBI with 670 nm or 830 nm R/NIR-IT did not result in improvements in motor or sensory function or lesion size at 7 days (P>0.05). Similarly, treatment of contusive SCI with 670 nm or 830 nm R/NIR-IT did not result in significant improvements in functional recovery or reduced cyst size at 28 days (P>0.05). Outcomes from this comparative study indicate that it will be necessary to optimise delivery devices, wavelength, intensity and duration of R/NIR-IT individually for different CNS injury types.

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Sigma-Aldrich
L -还原型谷胱甘肽, ≥98.0%
Sigma-Aldrich
L -还原型谷胱甘肽, suitable for cell culture, BioReagent, ≥98.0%, powder
Supelco
谷胱甘肽, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L -还原型谷胱甘肽, BioXtra, ≥98.0%
Sigma-Aldrich
二氢乙锭, ≥95%
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锰, powder, ≥99.9% trace metals basis
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二氢乙锭, BioReagent, suitable for fluorescence, ≥95% (HPCE)
谷胱甘肽, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
锰, powder, −325 mesh, ≥99% trace metals basis
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3-硝基-L-酪氨酸, crystalline
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锰, chips, thickness <2.0 mm, 99%
锰, foil, 25x25mm, thickness 2mm, hot-pressed, 99.95%
锰, foil, 50x50mm, thickness 2mm, hot-pressed, 99.95%
锰, foil, not light tested, 100x100mm, thickness 0.0125mm, permanent polyester support, 98.7%
锰, foil, not light tested, 100x100mm, thickness 0.01mm, permanent polyester support, 98.7%
锰, foil, not light tested, 100x100mm, thickness 0.025mm, permanent polyester support, 98.7%
锰, foil, not light tested, 100x100mm, thickness 0.02mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.001mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.0025mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.002mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.003mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.004mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.005mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.006mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.007mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.008mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.009mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.0125mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.01mm, permanent polyester support, 98.7%
锰, foil, not light tested, 25x25mm, thickness 0.025mm, permanent polyester support, 98.7%