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  • The binding affinity of phthalate plasticizers-protein revealed by spectroscopic techniques and molecular modeling.

The binding affinity of phthalate plasticizers-protein revealed by spectroscopic techniques and molecular modeling.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2014-07-06)
Yuanyuan Yue, Jianming Liu, Ren Liu, Yangyang Sun, Xiaoge Li, Jing Fan
摘要

Phthalate plasticizers have been subjected to close scrutiny and evidences of their toxicity and other negative environmental impacts have arisen as a result of their use in food in some countries. Once entering human body, plasticizers could affect the conformation of human serum albumin and protein function. The interaction between two phthalate plasticizers and human serum albumin was investigated by multispectroscopic techniques and molecular modeling. The alteration in protein conformational stability was determined by fluorescence quenching data. The thermodynamic parameters indicated that the hydrophobic interactions played a major role in the process. In addition, the alterations of HSA secondary structure in the presence of phthalate plasticizers were investigated. Molecular modeling and displacement experiments showed that phthalate plasticizers situated within subdomain IIA (site I) of HSA. Furthermore, the binding distances for the plasticizers-HSA system were provided by the efficiency of fluorescence resonance energy transfer.

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邻苯二甲酸二辛酯, ≥99.5%
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邻苯二甲酸二正辛酯, ≥98.0% (GC)
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邻苯二甲酸二正辛酯, analytical standard
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氟芬那酸, analytical standard, for drug analysis
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邻苯二甲酸二正辛酯, analytical standard
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锗, powder, −100 mesh, ≥99.99% trace metals basis
Supelco
邻苯二甲酸二正辛酯, certified reference material, TraceCERT®
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锗, microfoil, 25x25mm, thinness 0.5μm, specific density 333μg/cm2, 6 micron aluminum permanent support, 100%
锗, microfoil, 50x50mm, thinness 0.25μm, specific density 166.3μg/cm2, 6 micron aluminum permanent support, 100%
锗, rod, 25mm, diameter 2.0mm, polycrystalline, n-type, 99.999%
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锗, sheet, 25x25mm, thickness 1.0mm, polycrystalline, 99.999%
锗, sheet, 25x25mm, thickness 1.0mm, single crystal, 99.999%
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锗, sheet, 50x50mm, thickness 0.5mm, single crystal, 99.999%
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锗, sheet, 6x6mm, thickness 1.0mm, polycrystalline, 99.999%
锗, sheet, 7x24mm, thickness 1.0mm, polycrystalline, 99.999%