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Pinus densiflora Sieb. et Zucc. alleviates lipogenesis and oxidative stress during oleic acid-induced steatosis in HepG2 cells.

Nutrients (2014-07-25)
Yu-Jin Hwang, Hae-Ri Wi, Haeng-Ran Kim, Kye Won Park, Kyung-A Hwang
ABSTRACT

Excess accumulation of lipids and oxidative stress in the liver contribute to nonalcoholic fatty liver disease (NAFLD). We hypothesized that Pinus densiflora Sieb. et Zucc. (PSZ) can protect against NAFLD by regulating lipid accumulation and oxidative stress in the liver. To investigate the effect of PSZ upon NAFLD, we used an established cellular model: HepG2 cells treated with oleic acid. Then, the extent of hepatic steatosis and oxidative stress was assessed and levels of inflammatory markers measured. Oleic acid-treated HepG2 cells, compared with controls, had greater lipid accumulation. PSZ decreased lipid accumulation by 63% in oleic acid-treated HepG2 cells. Additionally, PSZ decreased the target gene expression of lipogenesis such as sterol regulatory element binding protein-1c, fatty acid synthase, stearoyl-CoA desaturase-1, diacylglycerol O-acyltransferase-1, and acetyl-CoA carboxylase-1 by 1.75, 6.0, 2.32, 1.93 and 1.81 fold, respectively. In addition, Oleic acid-treated HepG2 cells elicited extensive accumulation of tumor necrosis factor-α (TNFα) by 4.53 fold, whereas PSZ-treated cells decreased the expression of TNFα mRNA by 1.76 fold. PSZ significantly inhibited oxidative stress induced by reactive oxygen species. These results suggest that PSZ has effects on steatosis in vitro and further studies are needed in vivo to verify the current observations.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
油酸, ≥99% (GC)
Sigma-Aldrich
油酸, technical grade, 90%
Sigma-Aldrich
油酸, suitable for cell culture, BioReagent
Supelco
油酸, analytical standard
Sigma-Aldrich
油酸, natural, FCC
Sigma-Aldrich
硼, crystalline, 1 cm, 99.7% trace metals basis
Sigma-Aldrich
油酸, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)
油酸, European Pharmacopoeia (EP) Reference Standard
Supelco
油酸, Selectophore, ≥99.0%
硼, microfoil, disks, 10mm, thinness 0.1μm, specific density 23.5μg/cm2, permanent mylar 3.5μm support, 99.6%
硼, microfoil, disks, 25mm, thinness 0.1μm, specific density 23.5μg/cm2, permanent mylar 3.5μm support, 99.6%
硼, monofilament, 100m, diameter 0.1mm
硼, monofilament, 10m, diameter 0.1mm
硼, monofilament, 10m, diameter 0.2mm
硼, monofilament, 200m, diameter 0.1mm
硼, monofilament, 20m, diameter 0.1mm
硼, monofilament, 20m, diameter 0.2mm
硼, monofilament, 50m, diameter 0.1mm
硼, monofilament, 50m, diameter 0.2mm
硼, monofilament, 5m, diameter 0.1mm
硼, monofilament, 5m, diameter 0.2mm