Merck
  • 主页
  • 查找结果
  • Liquid Chromatographic Analysis of the Interaction between Amino Acids and Aromatic Surfaces Using Single-Wall Carbon Nanotubes.

Liquid Chromatographic Analysis of the Interaction between Amino Acids and Aromatic Surfaces Using Single-Wall Carbon Nanotubes.

Langmuir : the ACS journal of surfaces and colloids (2015-07-25)
Kazuki Iwashita, Kentaro Shiraki, Rieko Ishii, Takeshi Tanaka, Atsushi Hirano
摘要

Proteins have nonspecific adsorption capacities for solid surfaces. Although the nonspecific adsorption capacities are generally understood to be related to the hydrophobicity or charge density of the surfaces, little is known at the amino acid level about the interaction between proteins and polyaromatic surfaces such as carbon nanotubes, which have recently been used for biotechnology applications. In this study, we investigated the interaction between proteinogenic amino acids and carbon nanotubes using high-performance liquid chromatography on silica matrices coated by single-wall carbon nanotubes (SWCNTs). Among the amino acids used in this study, tryptophan, tyrosine, and phenylalanine showed exceptional affinity for the matrices. The characteristic affinities of these amino acids were ascribed to their unique interactions with the large polyaromatic surfaces of the SWCNTs. These results are useful for understanding and controlling protein adsorption onto aromatic surfaces.

材料
货号
品牌
产品描述

Sigma-Aldrich
1-甲基-2-吡咯烷酮, anhydrous, 99.5%
Sigma-Aldrich
甘氨酸, suitable for electrophoresis, ≥99%
Sigma-Aldrich
甘氨酸, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
柠檬酸, ACS reagent, ≥99.5%
Sigma-Aldrich
脱氧胆酸钠, ≥97% (titration)
Sigma-Aldrich
氢氧化钠 溶液, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
尿素, powder, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
硫酸铵, for molecular biology, ≥99.0%
Sigma-Aldrich
尿素, ACS reagent, 99.0-100.5%
Sigma-Aldrich
L-组氨酸, ReagentPlus®, ≥99% (TLC)
Sigma-Aldrich
色氨酸 -L, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
盐酸, 36.5-38.0%, BioReagent, for molecular biology
Sigma-Aldrich
盐酸 溶液, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
氢氧化钠 溶液, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
柠檬酸, 99%
Sigma-Aldrich
磷酸钠, 96%
Sigma-Aldrich
L-组氨酸, suitable for cell culture, meets EP, USP testing specifications, from non-animal source
Sigma-Aldrich
脱氧胆酸钠, BioXtra, ≥98.0% (dry matter, NT)
Sigma-Aldrich
甘氨酸, BioUltra, for molecular biology, ≥99.0% (NT)
Sigma-Aldrich
L-亮氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-异亮氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-异亮氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
尿素, ReagentPlus®, ≥99.5%, pellets
Sigma-Aldrich
L-亮氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-苯基丙氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-苯基丙氨酸, reagent grade, ≥98%
Sigma-Aldrich
色氨酸 -L, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Sigma-Aldrich
尿素 溶液, BioUltra, ~8 M in H2O
Sigma-Aldrich
L-丙氨酸, ≥98% (TLC)
Sigma-Aldrich
L -缬氨酸, reagent grade, ≥98% (HPLC)