Merck

Hydrogenophaga luteola sp. nov. isolated from reed pond water.

Antonie van Leeuwenhoek (2015-07-15)
Juan Du, Jung-Eun Yang, Hina Singh, Shahina Akter, KyungHwa Won, Chang Shik Yin, Feng-Xie Jin, Tae-Hoo Yi
ABSTRACT

A yellowish colored, Gram-staining negative, strictly aerobic, motile, rod-shaped bacterium, designated THG-SQE7(T), was isolated from reed pond water in Shangqiu, PR China. Comparative 16S rRNA gene sequence analysis indicated that strain THG-SQE7(T) is most closely related to Hydrogenophaga pseudoflava ATCC 33668(T) (98.4 %), followed by Hydrogenophaga bisanensis K102(T) (97.6 %) and Hydrogenophaga flava CCUG 1658(T) (97.6 %). DNA-DNA hybridization showed 53.5, 36.0 and 22.5 % DNA re-association with H. pseudoflava KCTC 2348(T), H. bisanensis KCTC 12980(T) and H. flava KCTC 1648(T), respectively. Chemotaxonomic data revealed that strain THG-SQE7(T) possesses ubiquinone-8 as the only isoprenoid quinone, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0 and C18:1 ω7c as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was determined to be 63.7 mol%. These data corroborated the affiliation of strain THG-SQE7(T) to the genus Hydrogenophaga. Thus, the isolate represents a novel species, for which the name Hydrogenophaga luteola sp. nov. is proposed, with THG-SQE7(T) as the type strain (=KCTC 42501(T) = CCTCC AB 2014314(T) = JCM 30433(T)).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
硫酸, 99.999%
Sigma-Aldrich
L-酪氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
曲古抑菌素A, ≥98% (HPLC), from Streptomyces sp.
Sigma-Aldrich
柠檬酸铁, BioReagent, suitable for cell culture
SAFC
L-酪氨酸
Sigma-Aldrich
L-酪氨酸, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Sigma-Aldrich
柠檬酸铁 (III), technical grade
Sigma-Aldrich
L-酪氨酸, BioUltra, ≥99.0% (NT)
Sigma-Aldrich
脱氧核糖核酸 钠盐 来源于大肠杆菌 菌株 B, Genomic, unsheared
SAFC
柠檬酸铁
Sigma-Aldrich
L-酪氨酸, FG