Merck
  • Home
  • Search Results
  • Impact of Precursors Creatine, Creatinine, and Glucose on the Formation of Heterocyclic Aromatic Amines in Grilled Patties of Various Animal Species.

Impact of Precursors Creatine, Creatinine, and Glucose on the Formation of Heterocyclic Aromatic Amines in Grilled Patties of Various Animal Species.

Journal of food science (2015-10-08)
Monika Gibis, Jochen Weiss
ABSTRACT

The impact of precursors such as creatine, creatinine, and glucose on the formation of mutagenic/carcinogenic heterocyclic amines (HAs) were studied in patties of 9 different animal species equally heat treated with a double-plate contact grill. All grilled patties of the various species (veal, beef, pork, lamb, horse, venison, turkey, chicken, ostrich) contained several HAs such as MeIQx (2-amino-3,8-dimethylimidazo[4,5-f] quinoxaline; 0.5-1.4 ng/g), 4,8-DiMeIQx (2-amino-3,4,8-trimethylimidazo[4,5-f] quinoxaline, 0 to 1.3 ng/g), PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, 1.2 to 10.5 ng/g), harman (1-methyl-9H-pyrido[3,4-b] indole; 0.5 to 3.2 ng/g), and/or norharman (9H-pyrido[3,4-b]indole 0.5 to 1.9 ng/g). Residual glycogen (glucose) content varied greatly from 0.07 to 1.46 wt% on a dry matter (DM) basis. Total creatin(in)e content in raw meat (1.36 to 2.0 wt% DM) hardly differed between species, except in turkey and ostrich (1.1 wt% DM). Chicken contained, compared to all other species, very low concentrations of glucose (0.07 wt% DM) and the highest levels of nonprotein nitrogen compounds. The free amino acids lysine (r = 0.77, P < 0.001), tyrosine, phenylalanine, proline, isoleucine, and aspartic acid (r = 0.47-0.56, P < 0.05) showed significant correlation to PhIP in chicken. Also a linear correlation was found to exist between PhIP (r = 0.87, P < 0.001) and MeIQx (r = 0.35, P < 0.01), and the molar ratio of creatin(in)e to glucose, respectively. Harman as co-mutagens was linearly correlated to the concentration of glucose (r = 0.65, P < 0.001). By contrast, norharman was not significant correlated to glucose levels.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
二氯甲烷, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
2-丙醇, BioReagent, for molecular biology, ≥99.5%
Sigma-Aldrich
乙腈, anhydrous, 99.8%
Sigma-Aldrich
甲醇, anhydrous, 99.8%
Sigma-Aldrich
2-丙醇, ACS reagent, ≥99.5%
Sigma-Aldrich
乙酸乙酯, ACS reagent, ≥99.5%
Sigma-Aldrich
盐酸, ACS reagent, 37%
Sigma-Aldrich
三乙基胺, ≥99.5%
Sigma-Aldrich
氢氧化钠, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
盐酸, ACS reagent, 37%
Sigma-Aldrich
甲醇, ACS reagent, ≥99.8%
Sigma-Aldrich
2-丙醇, anhydrous, 99.5%
Sigma-Aldrich
二乙醚, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
Sigma-Aldrich
氢氧化钾, ACS reagent, ≥85%, pellets
Sigma-Aldrich
磷酸, 85 wt. % in H2O, 99.99% trace metals basis
Sigma-Aldrich
氢氧化钠, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
二氯甲烷, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%
Sigma-Aldrich
乙酸乙酯, anhydrous, 99.8%
Sigma-Aldrich
高氯酸, ACS reagent, 70%
Sigma-Aldrich
氢氧化钠 溶液, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
二乙醚, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
三乙基胺, ≥99%
Sigma-Aldrich
咖啡因, powder, ReagentPlus®
Sigma-Aldrich
氢氧化钠 溶液, 50% in H2O
Sigma-Aldrich
磷酸, ACS reagent, ≥85 wt. % in H2O
Sigma-Aldrich
色氨酸 -L, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
氢氧化钠, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
Sigma-Aldrich
L-精氨酸, reagent grade, ≥98%
Sigma-Aldrich
盐酸, 36.5-38.0%, BioReagent, for molecular biology
Sigma-Aldrich
盐酸 溶液, 1.0 N, BioReagent, suitable for cell culture