[跳转至内容](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/SG/zh) 产品 购物车0 SGZH 产品 产品应用服务资源支持 [登录 / 注册](https://www.sigmaaldrich.com/oidc-sign-in) [订单查询](https://www.sigmaaldrich.com/SG/zh/order-lookup) [快速订购](https://www.sigmaaldrich.com/SG/zh/quick-order) 购物车0 [Home](https://www.sigmaaldrich.com/SG/en)[Solid Phase Microextraction (SPME)](https://www.sigmaaldrich.com/SG/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction)Manual SPME Sampling # Manual SPME Sampling [Learn More About SPME](https://www.sigmaaldrich.com/SG/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction) Beginners should consider using a manual SPME fiber and holder. The manual system allows the analyst to optimize the extraction process by evaluating different fibers and extraction conditions in a consistent manner. Begin the extraction process by placing the sample into the vial puck on the hotplate. The vial puck allows users to easily index the vial to begin sample agitation. Insert the holder into the sampling stand, passing the needle through the septum that seals the sample vial. Depress the plunger down to expose the fiber to the sample liquid or headspace above the sample. Start the timing of exposure of the SPME fiber for reproducible extractions. After a predetermined time, the SPME fiber is retracted into the protective needle and transferred to the GC for analysis. Inject the SPME fiber into the GC injection port, where the collected organics are quickly transferred to the GC column by heating the fiber. After the thermal transfer of analytes is completed, the fiber is removed and inserted in the next sample vial for extraction. __Related Articles__ - [Biodiesel](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/biodiesel) - [Automated Solid Phase Microextraction (SPME) Sampling](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/automated-spme-sampling) - [Bioanalysis with SPME](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/bioanalysis-with-spme) - [BioSPME for Plasma Protein Binding Assay: Details of Method Development and Optimization](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/biospme-plasma-protein-binding-assay) - [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) - [GC/MS/MS Analysis of BPA in Energy Drink](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/gc-ms-ms-analysis-of-bisphenol-a-bpa-in-a-fruit-flavored-energy-drink-cla010218a) - [Rugged SPME Sampling](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) - [SPME Fiber Performance Tips for Volatiles & Semi-Volatiles](https://www.sigmaaldrich.com/SG/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/performance-tips) - [View More](https://www.sigmaaldrich.com/SG/en/search/facet-search?focus=sitecontent&term=facet-search) 顶部 __登录以继续。__ 如要继续阅读，请登录或创建帐户。 登录__暂无帐户？__注册 支持[客户支持](https://www.sigmaaldrich.com/SG/zh/support/customer-support)[联系我们](https://www.sigmaaldrich.com/SG/zh/collections/offices)[常见问题](https://maestro.my.site.com/knowledgeportal/s/)[安全数据表（SDS）](https://www.sigmaaldrich.com/SG/zh/documents-search?tab=sds)[证书（COA/COO）](https://www.sigmaaldrich.com/SG/zh/documents-search?tab=coa)[质量与监管](https://www.sigmaaldrich.com/SG/zh/life-science/quality-and-regulatory-management)[计算器和应用程序](https://www.sigmaaldrich.com/SG/zh/support/calculators-and-apps)[网络研讨会](https://www.sigmaaldrich.com/SG/zh/collections/webinars) 订单[快速订购](https://www.sigmaaldrich.com/SG/zh/quick-order)[定制产品](https://www.sigmaaldrich.com/SG/zh/services/custom-products)[电子商务解决方案](https://www.sigmaaldrich.com/SG/zh/life-science/ecommerce/ecommerce-solutions) 公司名称[关于我们](https://www.sigmaaldrich.com/SG/zh/life-science/about-us)[责任](https://www.sigmaaldrich.com/SG/zh/life-science/ssbi)[活动](https://www.sigmaaldrich.com/SG/zh/collections/events)[新闻发布](https://www.sigmaaldrich.com/SG/zh/collections/press)[计划](https://www.sigmaaldrich.com/SG/zh/life-science/partnership-programs)[职业生涯](https://careers.merckgroup.com/global/en)[办事处](https://www.sigmaaldrich.com/SG/zh/collections/offices) 社交媒体 [](https://www.linkedin.com/company/merck-life-science) [](https://x.com/Merck_lifesci) [](https://www.facebook.com/Merck.lifescience) [](https://www.instagram.com/mercklifescience/) [](https://www.youtube.com/@MerckLifeScience/featured) Merck __Research.开发。__ 我们是全球生命科学行业的领先供应商，为研究、生物技术开发和生产以及药物治疗开发和生产提供解决方案和服务。 [](https://www.sigmaaldrich.com/SG/zh) [Sigma-Aldrich® 溶液](https://www.sigmaaldrich.com/SG/zh/life-science/sigma-aldrich)[BioReliance® 解决方案](https://www.sigmaaldrich.com/SG/zh/life-science/bioreliance)[密理博溶液](https://www.sigmaaldrich.com/SG/zh/life-science/millipore)[SAFC® 解决方案](https://www.sigmaaldrich.com/SG/zh/life-science/safc)[Milli-Q® 解决方案](https://www.sigmaaldrich.com/SG/zh/life-science/milliq)[Supelco® 解决方案](https://www.sigmaaldrich.com/SG/zh/life-science/supelco) © 2026 Merck KGaA, Darmstadt, Germany and/or its affiliates.版权所有，包括用于人工智能培训的文本和数据挖掘以及类似技术。 未经许可，严禁复制本网站的任何资料。 [网站使用条款](https://www.sigmaaldrich.com/SG/zh/life-science/legal/site-use-terms)|[隐私政策](https://www.sigmaaldrich.com/SG/zh/life-science/legal/privacy-statement)|[一般销售条款和条件](https://www.sigmaaldrich.com/SG/zh/life-science/legal/terms-and-conditions)|[版权同意书](https://www.sigmaaldrich.com/SG/zh/life-science/legal/copyright-consent)|[网站地图](https://www.sigmaaldrich.com/SG/zh/site-map) An unknown error has occured.