1.03854

GranuCult^® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar

acc. FDA-BAM, ISO 21872

• Synonym(s): TCBS Agar, Thiosulfate Citrate Bile Salts Sucrose Agar, Vibrio Selective Agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: ISO 21872
• Quality Level: 200
• form: powder
• technique(s): microbiological culture: suitable
• pH: 8.6±0.2 (25 °C)
• application(s): food and beverages; pharmaceutical
• storage temp.: 15-25°C
• suitability: selective and differential for Vibrio spp.

Description

General description

GranuCult^® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar is a complex medium designed for the isolation and differentiation of Vibrio spp. and other enteropathogenic microorganisms from products intended for human consumption, animal feed, environmental samples in the production area, food production, food handling, and other materials.
The synergistic effect of high concentration of thiosulfate, citrate and strong alkalinity inhibits the growth of Enterobacteriaceae spp. The medium is highly selective as the bile salts present in the medium suppresses the growth of Enterococci spp.
The differentiation is achieved based on sucrose fermentation, pH indicators and H₂S production from thiosulfate. Vibrio spp. do not produce H₂S but depending on the species, may ferment sucrose. Peptone and yeast extract in the media allow good growth and agar is the solidifying agent.

Application

GranuCult^® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar is used to isolate and differentiate Vibrio spp. and other enteropathogenic microorganisms from products intended for human consumption, animal feed, environmental samples in the production area, food production, food handling, and other materials.

Analysis Note

• The dehydrated medium is a granulate with green color.
• The prepared medium is clear to slightly opalescent and blue-green.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3