PPB006

Phosphate buffered saline

pH 7.4, pHast Pack^™, powder

• Synonym(s): 1X PBS wash buffer, PBS
• MDL number: MFCD00131855
• NACRES: NA.21

Properties

• product line: pHast Pack^™
• form: powder
• pH: 7.4
• foreign activity: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected

Description

General description

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Application

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

Features and Benefits

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable