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D2650

Sigma-Aldrich

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

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Synonym(s):
DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

grade

Hybri-Max

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

Assay

≥99.7%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable
cryopreservation: suitable

impurities

endotoxin, tested
≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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