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T5574
Terrific Broth
Liquid microbial growth medium
Synonym(s):
TB medium, Tartoff – Hobbs medium
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500 ML
€45.20
€45.20
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.85
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grade
Molecular Biology
description
Liquid Medium
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Related Categories
1 of 4
This Item | T9179 | L2542 | L3522 |
|---|---|---|---|
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - |
| form liquid | form powder | form liquid | form powder |
| sterility sterile; 0.2 μm filtered | sterility non-sterile | sterility sterile; 0.2 μm filtered | sterility non-sterile |
| application(s) microbiology | application(s) agriculture | application(s) microbiology | application(s) food and beverages |
| Quality Level 500 | Quality Level 200 | Quality Level 400 | Quality Level 200 |
General description
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
Terrific Broth liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Other Notes
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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The preparation and analysis of DNA for use in transgenic technology.
Auerbach, A.B., et al.
Advanced Protocols For Animal Transgenesis, 501-501 (2011)
Shani Leviatan et al.
The Journal of biological chemistry, 285(31), 23548-23556 (2010-06-08)
Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for
Shaheen Shojania et al.
Journal of virological methods, 164(1-2), 35-42 (2009-11-28)
The HIV-1 transactivator of transcription (Tat) is a protein essential for virus replication. Tat is an intrinsically disordered RNA-binding protein that, in cooperation with host cell factors cyclin T1 and cyclin-dependent kinase 9, regulates transcription at the level of elongation.
Andrey Sokolov et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(3), 969-974 (2010-01-19)
Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class
Ørjan Samuelsen et al.
Antimicrobial agents and chemotherapy, 52(8), 2905-2908 (2008-06-19)
Purified recombinant VIM-7 possesses efficient penicillinase and carbapenemase activities comparable to those of VIM-2. Cephalosporinase activity was variable and generally lower than those of VIM-1 and VIM-2. A homology model suggests that the VIM-7 Tyr-218 Phe substitution may be responsible
Protocols
General protocols for growth of competent cells in microbial medium.
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
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What are the alternatives for T5574?
What are the alternatives for T5574?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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