WGA4
GenomePlex® Single Cell Whole Genome Amplification Kit
Amplify genome of a single cell
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General description
Application
- to amplify the isolated DNA
- in whole genome amplification (WGA)
- to amplify the microdissected DNAs
- for the amplification of circulating tumor cell genomic DNA from metastatic castration-resistant prostate cancer cells
- for the detection of copy number variations (CNV) by single-cell low-coverage whole-genome sequencing (SLWGS) method
- Gel electrophoresis
- qPCR
- comparative genomic hybridization (CGH) microarray
- Short tandem repeats (STR) analysis
- single nucleotide polymorphism (SNP) analysis
Features and Benefits
- Highly yield and accuracy of DNA amplification within four hours
- Amplification of DNA from any source such as cancer cells, epithelial cells, lymphocytes, fibroblast amniotic cells, polycarbonate fixed cells, and plant cells
- A complete representation of the entire genome with minimal allele bias
- WGA DNA polymerase is suitable for use with downstream applications including gel electrophoresis, qPCR, comparative genomic hybridization (CGH) microarray, short term repeat (STR) analysis, and single nucleotide polymorphism (SNP) analysis, TaqMan® assays, and microsatellite analysis
Other Notes
Legal Information
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
Protocols
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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