If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
53827-U
Ascentis® Express C18 (2.7 μm) HPLC Column
L × I.D. 10 cm × 4.6 mm, pk of 1 ea
Synonym(s):
Core-shell (SPP) Fused Core C18 HPLC column
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 ea | Please contact Customer Service for Availability | €878.00 |
About This Item
€878.00
material
stainless steel column
Quality Level
agency
suitable for USP L1
product line
Ascentis®
feature
endcapped
packaging
pk of 1 ea
parameter
60 °C temp. range, 600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
10 cm × 4.6 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
C18 (octadecyl) phase
particle size
2.7 μm
pore size
90 Å
operating pH
2-9
separation technique
reversed phase
General description
Visit the Ascentis Express home page for more information on this new column technology.
Application
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| L × I.D. 10 cm × 4.6 mm | L × I.D. 5 cm × 1 mm | L × I.D. 15 cm × 4.6 mm | L × I.D. 10 cm × 1.5 mm |
| particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique - |
| matrix active group C18 (octadecyl) phase | matrix active group C18 (octadecyl) phase | matrix active group C18 (octadecyl) phase | matrix active group C18 (octadecyl) phase |
| technique(s) HPLC: suitable, LC/MS: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) - |
| pore size 90 Å | pore size 90 Å | pore size 90 Å | pore size 90 Å |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 53827-U | 04061838534316 |
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What solution should be used to wash the Ascentis C18, 2.7um particle column?
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The recommended cleaning solution for reversed phase C18 columns is typically a mixture of the organic solvent used in the mobile phase and water. To remove impurities, it is advisable to reverse the column flow. Begin by flushing the column with HPLC grade water to eliminate the buffer, followed by a wash with a 40:60 or 50:50 ratio of the mobile phase solvent (methanol or ACN).
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What is the carbon load for Ascentis® Express C18 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
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Can I use Ascentis Express on a UHPLC system?
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Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
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Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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Can I use Ascentis Express on any type of HPLC system?
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Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
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The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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