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Cloning and Expression

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Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Simplicon™ RNA Transfection and Electroporation Protocols
Simplicon™ RNA Transfection and Electroporation Protocols
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
pGEX Vectors
This page describes the characteristics of pGEX expression vectors used with Cytiva products.
OverExpress™ Electrocompetent Cells
OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.
Alkaline Phosphatase (AP) Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Dephosphorylation Procedures for DNA and Proteins
Dephosphorylation of DNA using Calf Intestinal Alkaline Phosphatase, Shrimp Alkaline Phospha, Bovine Intestinal Alkaline Phosphatase
Calcium Phosphate Transfection Kit Protocol
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
SIG10 Chemically Competent Cells
For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.
Competent Cell Protocols
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
BL21(DE3) Electrocompetent Cells
Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.
Recombinant Selection
Various DNA manipulation techniques from minipreps to gel electrophoresis are discussed for molecular biology experiments.
DNA Ligation Protocol
Cloning process, joining linear DNA into a vector, is crucial for biotechnological experiments, enabling DNA fragment recombinant technology.
Blue-White Select™ Screening Reagent
Blue/white color selection streamlines vector insert differentiation, enhancing cloning efficiency for molecular biologists.
x-tracta Gel Extraction Tool
A quick, clean and easy to use alternative to using a scalpel or razor blade for the extraction of DNA and RNA bands from agarose gels following gel electrophoresis.
Rapid DNA Ligation Kit Protocol
Rapid DNA Ligation Kit Protocol
Preparing Cells for Electroporation
Preparing Cells for Electroporation
Escort™ III Transfection Reagent Protocol
The product bulletin providin detailed use protocol for easy DNA transfection.
OverExpress™ Chemically Competent Cells
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Reverse Transcription Protocol
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
X-tremeGENE™ HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Restriction Enzyme Cloning Manual Buffer Recipes
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Co-transfection of Plasmid DNA
Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.
Universal Transfection Reagent Protocol
Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.