Primary cell culture

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In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Primary Cell Culture FAQ

Primary Cell Culture: Frequently Asked Questions (FAQs)

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Human Dermal Fibroblasts (HDF) Culture Protocol

This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

Human Bronchial Epithelial Cell line

Technical information for working with human bronchial epithelial cell line including thawing, subculturing and cryopreservation.

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Follicle Dermal Papilla Cells (HFDPC) Culture Protocol

Technical information for working with human follicle dermal papilla cells including thawing, subculturing and cryopreservation.

Human Lung Fibroblasts (HLF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

Rat Brain Microvascular Endothelial Cells (RBMVEC) Culture Protocol

Technical information for working with rat brain microvascular endothelial cells including thawing, subculturing and cryopreservation

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) Culture Protocol

Technical information for working with human pulmonary artery smooth muscle cells including thawing, subculturing and cryopreservation

Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol

Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation

Human Lung Microvascular Endothelial Cells (HLMVEC) Culture Protocol

Technical information for working with human lung microvascular endothelial cells including thawing, subculturing and cryopreservation

Primary Human Hepatocytes Culture Protocol

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Primary Human Spheroid-Qualified Plateable Hepatocyte Culture Protocol

Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.

Human Epidermal Melanocytes (HEM) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Mesenchymal Stem Cells & Growth Media

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Ficoll^® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Protocol for Preparation and Cultivation of PromoCell® Cryopreserved and Proliferating Cells

Protocol for the seeding and cultivation of primary cells received in cryopreserved or proliferating formats. Information on primary cell culture, cell handling and passaging.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Human Epidermal Keratinocytes (HEK) Culture Protocol

Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK

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