This kit has not been specifically validated for buccal swab samples. However, the kit has been optimized for a use with small sample quantities, 2–5 × 10^7 cells. The end user would have to determine suitability. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/747/586/mitoiso2pisrev0524-ms.pdf
MITOISO2
Mitochondria Isolation Kit
sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells
Synonym(s):
Mitochondrial Extraction Kit, Mitochondrial Purification Kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Pricing and availability is not currently available.
Quality Segment
usage
sufficient for 50 applications (2-5 x 107 cells)
technique(s)
ELISA: suitable, western blot: suitable
input
mammalian sample(s)
shipped in
dry ice
storage temp.
−20°C
General description
Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.
Application
Features and Benefits
- The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
- The isolated mitochondria will mostly have intact inner and outer membranes.
- Can be used to extract mitochondrial proteins for proteome analysis
Other Notes
- This product is for R&D use only, not for drug, household, or other uses.
- Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
- Use ultrapure water for the preparation of reagents.
- Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.
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This Item | |||
|---|---|---|---|
| description sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells | description sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues | description 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates) | description sufficient for 100 fluorometric tests (flow cytometry) |
| technique(s) ELISA: suitable, western blot: suitable | technique(s) fractionation: suitable | technique(s) protein staining: suitable, flow cytometry: suitable | technique(s) - |
| input mammalian sample(s) | input mammalian sample(s) | input - | input - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
| usage sufficient for 50 applications (2-5 x 107 cells) | usage sufficient for 50 assays (2 mL), sufficient for 10-20 g (animal tissue) | usage kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette) | usage sufficient for 100 fluorometric tests (flow cytometry) |
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Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
Flash Point (°F)
188.6 °F - closed cup
Flash Point (°C)
87 °C - closed cup
WGK
WGK 3
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CAN I USE THIS KIT FOR BUCCAL SWAB SAMPLES ALSO ?
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