The specific formulation of each component is considered proprietary. However, there is no albumin present in the PKH kit components.
MIDI26
PKH26 Red Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonym(s):
pkh membrane dye, red pkh
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.47
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packaging
pkg of 1 kit
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable
fluorescence
λex 551 nm; λem 567 nm (PKH26 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
1 of 4
This Item | MINI26 | PKH26GL | MIDI67 |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 490 nm; λem 502 nm (PKH67 dye) |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) cell analysis |
General description
This kit provides enough dye for up to 50 samples of 20 million cells each and enough diluent for up to 30 samples of 20 million cells each when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.
Application
This kit is for general cell membrane labeling, including for in vitro cell labeling, in vitro proliferation studies, and long term in vivo cell tracking.
Other Notes
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
CellVue is a registered trademark of Phanos Technologies
Kit Components Only
Product No.
Description
- Diluent C 60 mL
- PKH26 Cell Linker in Ethanol .2 mL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
Flash Point(F)
57.2 °F - closed cup
Flash Point(C)
14 °C - closed cup
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Mingli Han et al.
Cell death & disease, 11(1), 43-43 (2020-01-24)
Trastuzumab is commonly used in the treatment of human epidermal growth factor receptor-2 positive (HER-2+) breast cancer, but its efficacy is often limited by the emergence of chemoresistance. Recent studies indicate that exosomes act as vehicles for exchange of genetic
Lars T van der Veken et al.
Journal of immunology (Baltimore, Md. : 1950), 182(1), 92-101 (2008-12-26)
Killer Ig-like receptors (KIR) are expressed by human NK cells and T cells. Although Ag-specific cytolytic activity and cytokine production of KIR(+) T cells can be inhibited by KIR ligation, the effect of KIR on proliferation is unclear. KIR(+) T
Esther R van Bladel et al.
Blood, 123(10), 1556-1563 (2014-01-05)
Immune thrombocytopenia (ITP) is an autoimmune disease with a complex heterogeneous pathogenesis and a bleeding phenotype that is not necessarily correlated to platelet count. In this study, the platelet function was assessed in a well-defined cohort of 33 pediatric chronic
Andres Jerez et al.
Blood, 122(14), 2453-2459 (2013-08-09)
Large granular lymphocyte leukemia (LGL) is often associated with immune cytopenias and can cooccur in the context of aplastic anemia (AA) and myelodysplastic syndromes (MDS). We took advantage of the recent description of signal transducer and activator of transcription 3
Chuang Gao et al.
Aging, 11(24), 12147-12164 (2019-12-17)
Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The
Articles
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
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Is there any BSA used in this product?
1 answer-
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Is there any BSA in this product?
1 answer-
The diluent in the kit is isotonic. It does not contain BSA, detergent, organic solvent, and physiological salt.
Helpful?
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Is it possible to dual label RBCs with PKH dyes?
Is it possible to dual label RBCs with PKH dyes?
1 answer-
The following journal article may be relevant for your application: https://ashpublications.org/bloodadvances/article/2/20/2581/16117/Sensing-of-red-blood-cells-with-decreased-membrane
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